Carbohydrate availability modifies sorbitol dehydrogenase activity of apple fruit
Carbohydrate availability modifies sorbitol dehydrogenase activity of apple fruit
复制标题
DOI：
10.1034/j.1399-3054.1999.105301.x
复制
发表时间：
1999-03-01
影响因子：
6.4
通讯作者：
Archbold, DD
中科院分区：
文献类型：
--
作者：
Archbold, DD
对着单个苹果的茎或距完全去叶和韧皮部环剥（D/G）。开花后70天或更长时间的果实导致果实生长减少。D/G处理的果实皮层组织的可提取山梨醇脱氢酶（SDH）（酶代码，1.1.1.14）活性以及山梨醇和淀粉含量随后下降。对照和D/G皮质组织的GO提取产生预期水平的SDH活性，表明D/G果实中可提取的SDH活性的损失不是由于抑制剂或蛋白水解活性的存在。在缓冲的200 mM山梨糖醇或葡萄糖溶液中孵育D/G果实的皮质部分增加了可提取的SDH活性，并且在山梨糖醇溶液中孵育对照果实的皮质部分保持了活性。然而，无论是200 mM果糖或27 mM PEG，后者具有相同的渗透势的山梨醇溶液，影响D/G水果的可提取的SDH活性。结果表明，碳水化合物的可利用性可能会影响苹果果实的可提取SDH活性，而山梨醇和葡萄糖等特定碳水化合物可能是调节这种活性的信号。
Complete defoliation of the stem or spur and girdling the phloem (D/G) subtending a single apple (Malus domestica Borkh.) fruit at 70 or more days after bloom resulted in reduction of fruit growth. Extractable sorbitol dehydrogenase (SDH) (enzyme code, 1.1.1.14) activity and sorbitol and starch content of the cortex tissue of fruit receiving D/G treatment subsequently declined. Go-extraction of control and D/G cortex tissues yielded expected levels of SDH activity, indicating that the loss of extractable SDH activity in D/G fruit was not due to presence of an inhibitor or proteolytic activity. Incubating cortex sections from D/G fruit in a buffered 200 mM sorbitol or glucose solution increased extractable SDH activity, and incubating cortex sections from control fruit in the sorbitol solution maintained the activity. However,neither 200 mM fructose or 27 mM PEG, the latter with the same osmotic potential as the sorbitol solution, affected extractable SDH activity of D/G fruit. The results indicate that carbohydrate availability may affect extractable SDH activity of apple fruit, and that specific carbohydrates such as sorbitol and glucose may be signals for modulating this activity.