Isoproterenol decreases leptin expression in adipose tissue of obese humans.
Isoproterenol decreases leptin expression in adipose tissue of obese humans.
复制标题
异丙肾上腺素可降低肥胖人群脂肪组织中瘦素的表达。
DOI：
10.1002/j.1550-8528.1999.tb00401.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Fried,SK
中科院分区：
文献类型：
--
作者：
Ricci,MR;Fried,SK
利玛窦、马修·R. 和苏珊·K. 弗里德。异丙肾上腺素降低肥胖人体脂肪组织中的瘦素表达。Obes 研究目的：我们研究了非选择性 β 肾上腺素激动剂异丙肾上腺素 (Iso) 对人体脂肪组织中瘦素表达的影响。研究方法和程序：手术期间从 12 名病态肥胖受试者（10 名女性）中采集的皮下 (SQ) 和网膜脂肪 (OM) 组织和 2 名男性）在存在或不存在异丙肾上腺素 (10 μM) 的情况下，用胰岛素 (7 nM) 和/或地塞米松 (25 nM)（一种合成糖皮质激素）培养长达 24 小时。脂肪组织也在单独的含有或不含异丙肾上腺素的培养基中急剧培养3小时。测量了瘦素分泌和瘦素 mRNA 丰度。结果：在 OM 和 SQ 脂肪组织片段中，Iso 使瘦素释放急剧降低了 -30%（与无激素对照相比）。在 24 小时培养中，添加 Iso（在存在胰岛素的情况下）导致培养基中的瘦素积累降低（-20-30%），并且两个组织库中的瘦素 mRNA 水平（-40-50%）降低。与单独使用胰岛素相比，使用胰岛素和地塞米松进行培养可增加瘦素表达。添加 Iso 与胰岛素和地塞米松会降低介质瘦素 (−40–60%)，并且 24 小时后，来自两个储存库的经 Iso 处理的脂肪组织中的瘦素 mRNA 水平较低 (−65%)。培养 5 小时后未检测到异效应。讨论：我们得出的结论是，刺激 β-肾上腺素能受体可能通过两种机制调节人体脂肪组织中的瘦素表达：对瘦素释放的急性影响以及胰岛素和地塞米松对瘦素 mRNA 表达的刺激作用的长期拮抗作用。这些机制可能导致禁食期间血清瘦素的下降。
RICCI, MATTHEW R. AND SUSAN K. FRIED. Isoproterenol decreases leptin expression in adipose tissue of obese humans.Obes Res.Objective: We investigated the effects of the non‐selective β‐adrenergic agonist, isoproterenol (Iso), on leptin expression in human adipose tissue.Research Methods and Procedures: Subcutaneous (SQ) and omental adipose (OM) tissue taken during surgery from 12 morbidly obese subjects (10 women and 2 men) were cultured for up to 24 hours with insulin (7 nM) and/or dexamethasone (25 nM), a synthetic glucocorticoid, in the presence or absence of isoproterenol (10 μM). Adipose tissue was also acutely incubated for 3 hours in media alone with or without isoproterenol. Leptin secretion and leptin mRNA abundance were measured.Results: Iso acutely decreased leptin release by −30% (vs. no hormone controls) in fragments of OM and SQ adipose tissue. In 24‐hour culture, addition of Iso (in the presence of insulin) resulted in lower leptin accumulation in the medium (−20–30%) and leptin mRNA levels (−40–50%) from both tissue depots. Culture with insulin and dexamethasone increased leptin expression vs. insulin alone. Addition of Iso with insulin and dexamethasone decreased media leptin (−40–60%) and leptin mRNA levels were lower (−65%) in Iso‐treated adipose tissue from both depots after 24 hours. Iso effects were not detectable after 5 hours of culture.Discussion: We conclude that stimulation of β‐adrenergic receptors may modulate leptin expression in human adipose tissue by two mechanisms: an acute effect on leptin release and a longer‐term antagonism of stimulatory effects of insulin and dexamethasone on leptin mRNA expression. These mechanisms may contribute to the decline in serum leptin that occurs during fasting.