Multi-waveform fast-scan cyclic voltammetry mapping of adsorption/desorption kinetics of biogenic amines and their metabolites
Multi-waveform fast-scan cyclic voltammetry mapping of adsorption/desorption kinetics of biogenic amines and their metabolites
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DOI：
10.1039/c8ay00352a
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发表时间：
2018-06-28
影响因子：
3.1
通讯作者：
Jang, Dong Pyo
中科院分区：
文献类型：
--
作者：
Kim, Do Hyoung;Oh, Yoonbae;Jang, Dong Pyo
快速扫描循环伏安法（FSCV）是研究电活性神经化学物质的有效方法。近年来，FSCV在各种神经科学研究中被用于测量电活性神经递质。我们之前报道了配对脉冲伏安法（PPV）的使用，该方法利用被分析物在碳纤维微电极上的吸附特性，使FSCV能够区分各种分析物，并最大限度地减少混淆因素。尽管对神经递质的吸附/解吸特性进行了大量的研究，但神经递质之间吸附/解吸特性的差异尚未得到充分的探讨。为了计算神经递质的吸附/解吸常数，我们建议使用多波形FSCV (M-FSCV)，它由单次扫描中的十个三角形波形组成。在多个波形中，多巴胺的伏安响应由于吸附时间的缩短而呈指数衰减。利用吸附/解吸特性和从衰减方程中提取的指数衰减常数(K)和初始量(A)两个附加初始点，对衰减模式进行了数学描述。使用这种方法，我们能够量化衰减常数（K-map）和初始数量（a -map）颜色图，以及传统的伪颜色图。用两种生物胺（儿茶酚胺和吲哚胺）对M-FSCV进行了评估，以表征其固有的吸附/解吸常数。因此，a -map与浓度高度相关，K-map各组分化显著。这些结果表明，M-FSCV有潜力成为一种有用的技术，用于获取有关神经递质的额外吸附/解吸信息。
Fast-scan cyclic voltammetry (FSCV) is an effective method for investigating electro-active neurochemical species. In recent years, FSCV has been used to measure electro-active neurotransmitters in a variety of neuroscience studies. We previously reported on the use of paired-pulse voltammetry (PPV) that enables FSCV to differentiate various analytes and minimize confounding factors by taking advantage of the adsorption characteristics of the analyte on carbon fiber microelectrodes. In spite of a number of studies regarding adsorption/desorption characteristics of neurotransmitters, the difference in adsorption/desorption properties among neurotransmitters has yet to be fully explored. To calculate adsorption/desorption constants for neurotransmitters, we propose the use of multi-waveform FSCV (M-FSCV), which consists of ten triangular waveforms in a single scan. Within the multiple waveforms, the voltammetric response of dopamine decayed exponentially because of the decreased adsorption time period. The decay pattern was mathematically described using adsorption/desorption characteristics and two additional initial points: an exponential decay constant (K) and an initial quantity (A), which were extracted from the decay equation. Using this method, we were able to quantify the decay constant (K-map) and an initial quantity (A-map) color plot in addition to a conventional pseudo color plot. M-FSCV was evaluated with two biogenic amine groups (catecholamines and indolamines) to characterize their inherent adsorption/desorption constants. As a result, the A-map showed a high correlation with concentration and the K-map for each group to be significantly differentiated. These results demonstrate that M-FSCV has the potential to be a useful technique for acquiring additional adsorption/desorption information regarding neurotransmitters.