Dead bacterial biomass-assimilating bacterial populations in compost revealed by high-sensitivity stable isotope probing
Dead bacterial biomass-assimilating bacterial populations in compost revealed by high-sensitivity stable isotope probing
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DOI：
10.1016/j.envint.2019.105235
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发表时间：
2019-12-01
影响因子：
11.8
通讯作者：
Hori, Tomoyuki
中科院分区：
文献类型：
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作者：
Hanajima, Dai;Aoyagi, Tomo;Hori, Tomoyuki
已知病原体在堆肥中存活，并在某些因素的影响下再生，例如水分含量、温度和养分供应。死生物量通过提供可利用的养分，是可能影响病原体再生长的一个因素。然而，在堆肥的死生物质上生长的本土微生物，包括病原体，尚未被确定。在此，确定了致病指示细菌大肠杆菌在死亡细菌生物量存在下的再生长潜力，并确定了与大肠杆菌竞争生长的生物量代谢物。大肠杆菌的rRNA高灵敏度稳定同位素探针鉴定。培养依赖性分析表明，死亡细菌生物量的加入并没有刺激E。大肠杆菌生长。对来自用碳-13标记的死细菌生物质修正的堆肥样品的密度分辨的16 S rRNA分子的高通量分析揭示了死细菌同化细菌，包括鞘氨醇菌属，粘细菌谱系和芽孢杆菌目。这些细菌是潜在的竞争对手，由于其优先同化的死生物质堆肥的病原体。
Pathogens are known to survive in compost and to regrow under the influence of certain factors, such as moisture content, temperature and nutrient availability. Dead biomass, by providing available nutrients, is a factor that may affect pathogen regrowth. However, the indigenous microorganisms, including pathogens, that grown on the dead biomass of compost have not yet been identified. Here, the regrowth potential of the pathogenic indicator bacterium Escherichia coli in the presence of dead bacterial biomass was determined, and the biomass metabolizers that grew competitively with E. coli were identified by high-sensitivity stable isotope probing of rRNA. Culture-dependent analysis indicated that the addition of dead bacterial biomass did not stimulate E. coli growth. High-throughput analysis of density-resolved 16S rRNA molecules from compost samples amended with carbon-13-labeled dead bacterial biomass revealed dead bacterial-assimilating bacteria, including Sphingobium sp., myxobacterial lineages and Bacillales. These bacteria are potentially competitive with pathogens due to their preferential assimilation of dead biomass in compost.