Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model.
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model.
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DOI：
10.1590/1414-431x20165340
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发表时间：
2016-10-10
期刊：
影响因子：
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通讯作者：
Lima AA
中科院分区：
文献类型：
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作者：
Sampaio IC;Medeiros PH;Rodrigues FA;Cavalcante PA;Ribeiro SA;Oliveira JS;Prata MM;Costa DV;Fonseca SG;Guedes MM;Soares AM;Brito GA;Havt A;Moore SR;Lima AA
营养不良是中低收入国家面临的一项重大公共卫生挑战。本研究旨在评估巴西东北地区基础饲粮（RBD）是否会引起小鼠回肠的急性形态和功能变化。将瑞士小鼠（~ 25 g）分为两组：i)对照组小鼠喂食标准饮食，II)营养不良小鼠喂食RBD。7 d后处死小鼠，收集回肠，测定肠转运体和紧密连接的电生理参数（Ussing chambers）、转录（RT-qPCR）和蛋白表达（western blotting）。营养不良组的体重增加明显减少，隐窝深度减少，但绒毛高度没有变化。电生理测量显示，营养不良组的基础短路电流（I sc）降低，上皮阻抗无差异。特异性底物诱发的与亲和力和功效相关的I sc（谷氨酰胺和丙氨酰-谷氨酰胺）在各组间无显著差异，但葡萄糖诱导的I sc（功效）最大。营养不良组Sglt1和Pept1转录显著升高，SN-2转录降低。CAT-1和CFTR转录未见变化，而claudin-2和occludin转录在营养不良组显著升高。尽管mRNA有变化，但SGLT-1、PEPT-1、claudin-2和occludin蛋白的表达在两组间无差异。这些结果证明了RBD对小鼠的早期影响，包括在绒毛形态、肠道转运蛋白和紧密连接表达没有显著改变的情况下减轻体重和隐窝深度。
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no alterations in villus height. Electrophysiology measurements showed a reduced basal short circuit current (I sc) in the undernourished group, with no differences in transepithelial resistance. Specific substrate-evoked I sc related to affinity and efficacy (glutamine and alanyl-glutamine) were not different between groups, except for the maximum I sc (efficacy) induced by glucose. Transcription of Sglt1 and Pept1 was significantly higher in the undernourished group, while SN-2 transcription was decreased. No changes were found in transcription of CAT-1 and CFTR, while claudin-2 and occludin transcriptions were significantly increased in the undernourished group. Despite mRNA changes, SGLT-1, PEPT-1, claudin-2 and occludin protein expression showed no difference between groups. These results demonstrate early effects of the RBD on mice, which include reduced body weight and crypt depth in the absence of significant alterations to villus morphology, intestinal transporters and tight junction expression.