Fusion Peptide-Based Biomacromolecule Delivery System for Plant Cells
Fusion Peptide-Based Biomacromolecule Delivery System for Plant Cells
复制标题
基于融合肽的植物细胞生物大分子递送系统
DOI：
10.1021/acsbiomaterials.1c00227
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Numata Keiji
中科院分区：
文献类型：
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作者：
Watanabe Kenta;Odahara Masaki;Miyamoto Takaaki;Numata Keiji
随着基因组编辑等创新技术的发展，将DNA、RNA和蛋白质引入植物细胞在植物科学中变得非常重要。具有多个功能域的融合肽作为一种新的生物大分子递送方法已经被开发出来。功能域包括用于穿越细胞膜的细胞穿透肽，用于生物大分子结合的多阳离子肽和细胞器靶向肽。基于融合肽的大分子传递系统能够有效地将比肽大得多的DNA、RNA和蛋白质引入植物细胞，同时保持生物大分子的活性。与现有的输送方法相比，该系统的优点在于它不需要任何特殊设备，可以在各种植物上轻松快速地进行。此外，作为融合肽系统的一个特征，将细胞器靶向肽应用于融合肽可以选择性地将生物大分子递送到叶绿体或线粒体。在这里，我们提供了一种基于融合肽的生物大分子传递系统的代表性方法，并举例说明了生物大分子传递作为植物生物学和生物技术的新工具的结果。
The introduction of DNA, RNA, and proteins into plant cells has become important in plant science with the recent development of innovative technologies such as genome editing. As a new method for the delivery of such biomacromolecules, fusion peptides, which have multiple functional domains, have been developed. The functional domains include cell-penetrating peptides for crossing cell membranes, polycationic peptides for biomacromolecule binding, and organelle-targeting peptides. The fusion peptide-based macromolecule delivery system enables the efficient introduction of DNA, RNA, and proteins, which are much larger in size than the peptide, into plant cells while retaining the activity of the biomacromolecules. Compared to pre-existing delivery methods, this system has advantages in that it does not require any special equipment and can be performed easily and quickly on a wide variety of plants. Furthermore, as a characteristic feature of the fusion peptide system, the application of organelle-targeting peptides to fusion peptides allows selective delivery of biomacromolecules to chloroplasts or mitochondria. Here, we provide a representative method of the fusion peptide-based biomacromolecule delivery system and an example of the results of biomacromolecule delivery as promising new tools for plant biology and biotechnology.