The role of toxic planocerid flatworm larvae on tetrodotoxin accumulation in marine bivalves
The role of toxic planocerid flatworm larvae on tetrodotoxin accumulation in marine bivalves
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有毒扁虫幼虫对海洋双壳类河豚毒素积累的作用
DOI：
10.1016/j.aquatox.2021.105908
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发表时间：
2021
影响因子：
4.5
通讯作者：
Itoi Shiro
中科院分区：
文献类型：
--
作者：
Okabe Taiki;Saito Rion;Yamamoto Kohei;Watanabe Riku;Kaneko Yoshiki;Yanaoka Mutsumi;Furukoshi Seika;Yasukawa Shino;Ito Masaaki;Oyama Hikaru;Suo Rei;Suzuki Miwa;Takatani Tomohiro;Arakawa Osamu;Sugita Haruo;Itoi Shiro
河豚毒素（Tetrodotoxin，TTX）又称河豚毒素，是世界范围内海洋食用贝类中普遍存在的一种毒素。本研究以采集自日本北方群岛太平洋一侧的几种双壳类（Azumapecten farrerisubsp.akazara、Patinopecten yessoensi和Mytilus galloprovincialis）为材料，研究了在有毒浮游动物幼虫存在下TTX的积累。LC-MS/MS分析表明，TTX仅在A. farrerisubsp.akazara.毒性扁虫特异性PCR和直接测序结果表明，在冷冻后的双壳贝类消化道内容物中检测到多触浮游虫COI基因的DNA片段。在环境海水中也发现了扁角类幼虫。在水族箱中的毒性实验表明，贻贝M。galloprovincialis也通过进食有毒的扁形虫幼虫而被消灭。这些结果表明，TTX积累的来源是有毒的扁形虫幼虫的食用双壳类。
Tetrodotoxin (TTX), also known as pufferfish toxin, has been detected in marine edible bivalves worldwide. In this study, several bivalve species,Azumapecten farrerisubsp.akazara, Patinopecten yessoensisandMytilus galloprovincialis, collected from the Pacific side of the northern Japanese Islands, were studied for the accumulation of TTX in the presence of toxic planocerid larvae. LC-MS/MS analysis demonstrated that TTX was detected only in the midgut gland ofA. farrerisubsp.akazara. Toxic flatworm-specific PCR and direct sequencing of the amplicons showed that the DNA fragments of thePlanocera multitentaculata COIgene were detected in the gut contents of the toxified bivalves. The planocerid larvae were also detected in the environmental seawaters. Toxification experiments in the aquarium demonstrated that the musselM. galloprovincialiswas also toxified by feeding on the toxic flatworm larvae. These results suggest that the source of TTX accumulation in edible bivalves is toxic flatworm larvae.