SYNTHETIC TRIMER AND TETRAMER OF 3-BETA-D-RIBOSE-(1-1)-D-RIBITOL-5-PHOSPHATE CONJUGATED TO PROTEIN INDUCE ANTIBODY-RESPONSES TO HAEMOPHILUS-INFLUENZAE TYPE-B CAPSULAR POLYSACCHARIDE IN MICE AND MONKEYS
SYNTHETIC TRIMER AND TETRAMER OF 3-BETA-D-RIBOSE-(1-1)-D-RIBITOL-5-PHOSPHATE CONJUGATED TO PROTEIN INDUCE ANTIBODY-RESPONSES TO HAEMOPHILUS-INFLUENZAE TYPE-B CAPSULAR POLYSACCHARIDE IN MICE AND MONKEYS
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DOI：
10.1128/iai.60.5.1826-1833.1992
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发表时间：
1992-05-01
影响因子：
3.1
通讯作者：
POOLMAN, JT
中科院分区：
文献类型：
--
作者：
PEETERS, CCAM;EVENBERG, D;POOLMAN, JT
从b型流感嗜血杆菌荚膜多糖(PRP)中合成的寡糖通过硫醚键偶联到载体蛋白上。结合物由三聚体和四聚体核糖-核糖醇-磷酸和破伤风类毒素或白喉毒素制成。在成年小鼠和猴子中，所有结合物均可通过增加免疫球蛋白G/免疫球蛋白M的比例来诱导抗PRP抗体应答。与四聚体结合物相比，三聚体结合物诱导的抗PRP抗体应答较低。成年猴子对四聚寡糖-破伤风类毒素结合物的反应与寡糖-CRM197结合物(HBOC)一样好，这种结合物在注射两到三次后会在人类婴儿中产生保护性水平的血清抗体。
Synthetic oligosaccharides derived from the capsular polysaccharide (PRP) of Haemophilus influenzae type b were conjugated to carrier proteins via a thioether linkage. Conjugates were made of trimeric and tetrameric ribose-ribitol-phosphate and tetanus toxoid or diphtheria toxin. All conjugates elicited anti-PRP antibody responses with an increasing immunoglobulin G/immunoglobulin M ratio in adult mice and monkeys. Trimer conjugates elicited lower anti-PRP antibody responses compared with tetramer conjugates. Adult monkeys responded equally well to the tetrameric oligosaccharide-tetanus toxoid conjugate as to the oligosaccharide-CRM197 conjugate (HbOC), which elicits protective levels of serum antibodies in human infants after two or three injections.