Electro-optical BLM chips enabling dynamic imaging of ordered lipid domains.
Electro-optical BLM chips enabling dynamic imaging of ordered lipid domains.
复制标题
电光 BLM 芯片可实现有序脂质域的动态成像。
DOI：
10.1039/c2lc40077d
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发表时间：
2012
期刊：
影响因子：
6.1
通讯作者：
DeVoe,DonL
中科院分区：
文献类型：
--
作者：
Shao,Chenren;Kendall,EricL;DeVoe,DonL
对脂筏、细胞膜内鞘脂和胆固醇的有序微域的研究对于探索膜组织和细胞功能之间的关系至关重要。虽然脂相分离的体外研究通常使用球形囊泡作为模型膜进行，但这些模型的实用性受到许多因素的限制。在这里，我们提出了一种微流体设备，支持同时对片上双层脂膜（BLM）进行电测量和共焦成像，从而实现膜组织的实时多域成像。该芯片进一步支持膜两侧的封闭微流体通路，允许快速改变膜边界条件，并提供通过应用跨膜压力梯度动态调整膜曲率的机制。在这里，我们通过研究当膜成分往返于含有溶剂化脂质和胆固醇的支撑环时，有序脂质域的动态生成和溶解来展示该平台。
Studies of lipid rafts, ordered microdomains of sphingolipids and cholesterol within cell membranes, are essential in probing the relationships between membrane organization and cellular function. While in vitro studies of lipid phase separation are commonly performed using spherical vesicles as model membranes, the utility of these models is limited by a number of factors. Here we present a microfluidic device that supports simultaneous electrical measurements and confocal imaging of on-chip bilayer lipid membranes (BLMs), enabling real-time multi-domain imaging of membrane organization. The chips further support closed microfluidic access to both sides of the membrane, allowing the membrane boundary conditions to be rapidly changed and providing a mechanism for dynamically adjusting membrane curvature through application of a transmembrane pressure gradient. Here we demonstrate the platform through the study of dynamic generation and dissolution of ordered lipid domains as membrane components are transported to and from the supporting annulus containing solvated lipids and cholesterol.