IMIRS: a high-resolution 3D reconstruction package integrated with a relational image database
IMIRS: a high-resolution 3D reconstruction package integrated with a relational image database
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IMIRS：与关系图像数据库集成的高分辨率 3D 重建包
DOI：
10.1016/s1047-8477(02)00014-x
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发表时间：
2002-03-01
影响因子：
3
通讯作者：
Zhou, ZH
中科院分区：
文献类型：
--
作者：
Liang, YY;Ke, EY;Zhou, ZH
电子低温显微镜仪器和单粒子重建的最新进展为高通量和高分辨率的大分子复合物的三维（3D）结构测定创造了机会。然而，依靠传统的文本文件数据管理和命令行程序来组织和处理高分辨率研究所需的越来越多的图像数据已经变得不切实际和效率低下。在这里，我们提出了一个分布式的关系数据库管理复杂的数据集和它的集成到我们的高分辨率软件包IMIRS（图像管理和二十面体重建系统）。IMIRS由一整套用于二十面体重建的模块化程序组成，这些程序在图形用户界面下组织，并提供用户友好的分步数据处理以及自动重建选项。我们发现，IMIRS中的数据管理与处理的集成自动化繁琐的数据管理任务，使数据一致性，并促进信息共享在分布式计算机和用户环境中，而不会显着增加程序执行的时间。我们证明了IMIRS在二十面体重建向高分辨率的适用性，通过使用它来获得一个8埃的3D结构的中等大小的dsRNA病毒。(C)2002 Elsevier Science（美国）。All rights reserved.
Recent advances in electron cryomicroscopy instrumentation and single particle reconstruction have created opportunities for high-throughput and high-resolution three-dimensional (3D) structure determination of macromolecular complexes. However, it has become impractical and inefficient to rely on conventional text file data management and command-line programs to organize and process the increasing numbers of image data required in high-resolution studies. Here, we present a distributed relational database for managing complex datasets and its integration into our high-resolution software package IMIRS (Image Management and Icosahedral Reconstruction System). IMIRS consists of a complete set of modular programs for icosahedral reconstruction organized under a graphical user interface and provides options for user-friendly, step-by-step data processing as well as automatic reconstruction. We show that the integration of data management with processing in IMIRS automates the tedious tasks of data management, enables data coherence, and facilitates information sharing in a distributed computer and user environment without significantly increasing the time of program execution. We demonstrate the applicability of IMIRS in icosahedral reconstruction toward high resolution by using it to obtain an 8-Angstrom 3D structure of an intermediate-sized dsRNA virus. (C) 2002 Elsevier Science (USA). All rights reserved.