A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.
A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.
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CRISPR-Cas9 诱导的蝾螈突变实用指南。
DOI:
10.1007/978-1-0716-2659-7_22
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发表时间:
2023
期刊:
影响因子:
--
通讯作者:
Whited,JessicaL
中科院分区:
文献类型:
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作者:
Sousounis,Konstantinos;Courtemanche,Katharine;Whited,JessicaL
Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.