A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.

A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.
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CRISPR-Cas9 诱导的蝾螈突变实用指南。

DOI:
10.1007/978-1-0716-2659-7_22
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发表时间:
2023
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Whited,JessicaL
Whited,JessicaL
中科院分区:
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文献类型:
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作者:
Sousounis,Konstantinos;Courtemanche,Katharine;Whited,JessicaL

文献摘要

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聚集规则间隔短回文重复序列(CRISPR)是一种强大的工具,可以编辑蝾螈基因组。在本章中,我们将介绍如何检索基因序列,确认注释,设计CRISPR靶标,分析索引以及筛选CRISPR蝾螈。这是一个关于如何在你最喜欢的基因上使用CRISPR并深入了解其功能的综合指南。
Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.