Differential processing of subunits of human chorionic gonadotropin by granulosa cells in vivo.
Differential processing of subunits of human chorionic gonadotropin by granulosa cells in vivo.
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体内颗粒细胞对人绒毛膜促性腺激素亚基的差异加工。
DOI：
10.1073/pnas.77.8.4793
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发表时间：
1980
影响因子：
11.1
通讯作者：
MidgleyJr,AR
中科院分区：
文献类型：
--
作者：
Campbell,KL;Landefeld,TD;MidgleyJr,AR
通过使用双标记程序解决了糖蛋白激素人绒毛膜促性腺激素 (hCG) 的两个亚基是单独处理还是作为一个单元由靶细胞处理的问题。 hCG 的各个 α 和 β 亚基用 131I 或 125I 标记、重组、色谱分析，并静脉注射到激素引发的未成熟大鼠体内。这些大鼠的卵巢含有大量窦卵泡，其颗粒细胞具有高浓度的 hCG 受体。比较不同放射性同位素标记在不同组织中随时间的分布表明，与β亚基相关的活性优先被卵巢颗粒细胞以激素特异性方式保留，而与α亚基相关的活性优先丧失。这与其他组织形成对比，包括具有 hCG 受体的其他卵巢细胞，其中两种放射性标记要么被非差异性处理，要么以激素非特异性方式差异性处理。
The question of whether the two subunits of the glycoprotein hormone human chorionic gonadotropin (hCG) are handled separately or as a unit by target cells was addressed by using a dual-labeling procedure. The individual alpha and beta subunits of hCG were labeled with 131I or 125I, recombined, chromatographed, and injected intravenously into hormonally primed immature rats. The ovaries of these rats contained large numbers of antral follicles, the granulosa cells of which possess high concentrations of receptor for hCG. Comparisons of the distribution of the different radioisotopic labels in various tissues over time indicated the activity associated with the beta subunit was preferentially retained by the ovarian granulosa cells in a hormone-specific manner, while the activity associated with the alpha subunit was preferentially lost. This contrasted with other tissues, including other ovarian cells with receptor for hCG, in which both radiolabels were either handled nondifferentially or handled differentially in a hormonally nonspecific manner.