THE RATES OF FAST REACTIONS OF CARBON-DIOXIDE AND BICARBONATE IN HUMAN-ERYTHROCYTES MEASURED BY C-13 NMR
THE RATES OF FAST REACTIONS OF CARBON-DIOXIDE AND BICARBONATE IN HUMAN-ERYTHROCYTES MEASURED BY C-13 NMR
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DOI：
10.1016/0006-291x(87)90630-9
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发表时间：
1987-02-27
影响因子：
3.1
通讯作者：
HENKENS, RW
中科院分区：
文献类型：
--
作者：
HOFFMAN, DW;HENKENS, RW
应用碳-13核磁共振波谱技术研究人红细胞悬液中二氧化碳的毫秒级反应动力学。细胞内酶催化的CO2水合速率和.**图形 **。脱水的定量测定，以及CO2扩散进入和离开红细胞的速率。该方法还提供了在pH 6.0至pH 7.0范围内的细胞内pH的准确测量。温度依赖性的研究被用来确定细胞内水合-脱水反应的热力学函数。
The application of carbon-13 nuclear magnetic resonance spectroscopy to the study of the kinetics of millisecond timescale reactions of CO2 in human erythrocyte suspensions is described. The rates of intracellular enzyme catalyzed CO2 hydration and .**GRAPHIC**. dehydration were quantitatively determined, as well as the rates of CO2 diffusion into and out of the erythrocytes. The method also provides an accurate measure of the intracellular pH in the range of pH 6.0 to pH 7.0. A temperature dependence study was used to determine the thermodynamic functions for the intracellular hydration-dehydration reaction.