Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins
Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins
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DOI：
10.1080/03008200390152061
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发表时间：
2003-01-01
影响因子：
2.9
通讯作者：
Fedarko, NS
中科院分区：
文献类型：
--
作者：
Fisher, LW;Fedarko, NS
骨唾液蛋白（BSP）、牙本质基质蛋白1（DMPI）、牙本质唾液磷蛋白（DSPP）、釉蛋白（ENAM）、基质细胞外磷酸糖蛋白（MEPE）和骨桥蛋白（OPN）是在骨和/或牙齿中表达的糖磷蛋白。对它们的氨基酸序列的直接比较并不表明它们属于一个单一的遗传家族，但对它们的染色体位置和基因结构的详细分析却表明了这一点。通过RT-PCR分析人脑mRNA，发现了两个额外的外显子，从而使MEPE是SIBLING（小整合素结合配体，N-连接糖蛋白）家族的成员更加令人信服。我们建议，这个兄弟家族的成员是扩展的，灵活的蛋白质在溶液中，可以促进形成许多不同的复合物。例如，OPN可以将补体因子H桥连到RGD依赖性整联蛋白或CD 44上，形成主动抑制替代补体途径的膜结合复合物。两种可能的机制，抑制溶解途径的替代补体。
Bone sialoprotein (BSP), dentin matrix protein 1 (DMPI), dentin sialophosphoprotein (DSPP), enamelin (ENAM), matrix extracellular phosphoglycoprotein (MEPE), and osteopontin (OPN) are glycophosphoproteins expressed in bones and/or teeth. Direct comparison of their amino acid sequences do not suggest that they belong to a single genetic family, but a detailed analysis of their chromosomal location and gene structure does. Analysis of human brain mRNA by RT-PCR has led to the discovery of two additional exons thereby making it more convincing that MEPE is a member of the SIBLING (Small Integrin-Binding LIgand, N-linked Glycoprotein) family. We propose that the members of this SIBLING family are extended, flexible proteins in solution that can facilitate the formation of a number of different complexes. For example, OPN can bridge complement Factor H to either an RGD-dependent integrin or to CD44 forming a membrane-bound complex that actively suppresses the alternate complement pathway. Two possible mechanisms for inhibiting the lytic pathway of alternate complement are presented.