Noninvasive measurement of midexpiratory flow indicates bronchoconstriction in allergic rats
Noninvasive measurement of midexpiratory flow indicates bronchoconstriction in allergic rats
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DOI：
10.1152/japplphysiol.01121.2001
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发表时间：
2002-10-01
影响因子：
3.3
通讯作者：
Fabel, H
中科院分区：
文献类型：
--
作者：
Glaab, T;Hoymann, HG;Fabel, H
本研究旨在评价潮式呼吸模式分析评估清醒大鼠支气管收缩的价值和适用性。使用无创性，头向外的身体体积描记术和减少的潮气中呼气流量（EF 50），我们测量气道反应性（AR）吸入乙酰胆碱和过敏原在清醒的布朗-挪威大鼠，然后有创性测定肺电导（GL）和EF 50在麻醉大鼠。对乙酰胆碱的剂量反应研究表明，无创记录的EF 50密切反映了同时测量的GL和EF 50的有创监测所观察到的剂量依赖性降低。在致敏和对卵清蛋白或盐水的肠内加强后，评估大鼠对雾化卵清蛋白的早期和晚期AR。卵白蛋白气雾剂激发导致过敏原致敏大鼠出现早期和晚期AR，而对照组无反应。通过EF 50非侵入性测量的过敏原特异性AR与侵入性记录的EF 50和GL的程度相似，并且与IgE增强和气道炎症相关。我们的结论是，EF 50是一个非侵入性的和生理上有效的指标支气管收缩在大鼠哮喘模型。
This study was designed to evaluate the value and applicability of tidal breathing pattern analysis to assess bronchoconstriction in conscious rats. Using noninvasive, head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we measured airway responsiveness (AR) to inhaled acetylcholine and allergen in conscious Brown-Norway rats, followed by invasive determination of pulmonary conductance (GL) and EF50 in anesthetized rats. Dose-response studies to acetylcholine showed that noninvasively recorded EF50 closely reflected the dose-dependent decreases observed with the invasive monitoring of simultaneously measured GL and EF50. After sensitization and intratracheal boost to ovalbumin or saline, rats were assessed for early and late AR to aerosolized ovalbumin. Ovalbumin aerosol challenge resulted in early and late AR in allergen-sensitized rats, whereas controls were unresponsive. The allergen-specific AR, as measured noninvasively by EF50, was similar in degree compared with invasively recorded EF50 and GL and was associated with enhanced IgE and airway inflammation. We conclude that EF50 is a noninvasive and physiologically valid index of bronchoconstriction in a rat model of asthma.