Construction of a bacterial autoinducer detection system in mammalian cells.
Construction of a bacterial autoinducer detection system in mammalian cells.
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DOI：
10.1251/bpo98
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发表时间：
2004
影响因子：
6.4
通讯作者：
Rumbaugh KP
中科院分区：
文献类型：
--
作者：
Shiner EK;Reddy S;Timmons C;Li G;Williams SC;Rumbaugh KP
群体感应（Quorum sensing，QS）是一种细胞密度依赖性信号系统，被细菌用来协调群体内的基因表达。革兰氏阴性菌中的QS系统由LuxR家族的转录因子及其酰基高丝氨酸内酯（阿勒）配体组成。我们在这里描述了一种方法，用于检查QS信号系统在哺乳动物细胞中，使用工程LuxR型蛋白的机会致病菌，铜绿假单胞菌，它可以作为AHL依赖性转录因子。工程化的蛋白质响应于它们的同源配体并显示序列特异性DNA结合特性。该系统具有几个潜在的生物技术和生物应用。它可用于表征任何LuxR型蛋白，筛选动物和植物细胞提取物或渗出物中模拟或干扰阿勒信号传导的化合物，或筛选不同细胞类型的阿勒失活活性。
Quorum sensing (QS) is a cell density-dependent signaling system used by bacteria to coordinate gene expression within a population. QS systems in Gram negative bacteria consist of transcription factors of the LuxR family and their acyl homoserine lactone (AHL) ligands. We describe here a method for examining QS signaling systems in mammalian cells that uses engineered LuxR-type proteins from the opportunistic pathogen, Pseudomonas aeruginosa, which can function as AHL-dependent transcription factors. The engineered proteins respond to their cognate ligands and display sequence specific DNA binding properties. This system has several potential biotechnological and biological applications. It may be used to characterize any LuxR-type protein, screen animal and plant cell extracts or exudates for compounds that mimic or interfere with AHL signaling or to screen different cell types for AHL inactivating activities.