Construction of a genetic linkage map for roses using RAPD and AFLP markers
Construction of a genetic linkage map for roses using RAPD and AFLP markers
复制标题
DOI：
10.1007/s001220051310
复制
发表时间：
1999-09-01
影响因子：
5.4
通讯作者：
Mattiesch, L
中科院分区：
文献类型：
--
作者：
Debener, T;Mattiesch, L
二倍体月季杂交体的分离群体 (2n = 2x = 14) 用于构建月季基因组的第一个连锁图谱。基于所谓的“双假测交”设计，在 60 株 F-1 植物群体中总共分析了 305 个 RAPD 和 AFLP 标记。这些标记中的 278 个可以位于两个图谱的 14 个连锁群上，分别覆盖图谱总长度 326 和 370 cM。 93/1-117 和 93/1-119 的图中标记之间的平均距离分别为 2.4 和 2.6 cM。除了分子标记之外，控制两个表型性状、花瓣数量（双花与单花）和花颜色（粉红色与白色）的基因分别被映射到连锁群 3 和 2 上。最接近双花基因 Blfo 和最接近粉红色花颜色基因 Blfa 的标记在没有重组的情况下共分离。这些图谱为进一步对园艺重要基因（例如抗性基因）进行遗传分析提供了工具，并为玫瑰标记辅助育种提供了起点。
A segregating population of diploid rose hybrids (2n = 2x = 14) was used to construct the first linkage maps of the rose genome. A total of 305 RAPD and AFLP markers were analysed in a population of 60 F-1 plants based on a so-called ''double-pseudotestcross'' design. Of these markers 278 could be located on the 14 linkage groups of the two maps, covering total map lengths of 326 and 370 cM, respectively. The average distances between markers in the maps for 93/1-117 and 93/1-119 is 2.4 and 2.6 cM, respectively. In addition to the molecular markers, genes controlling two phenotypic characters, petal number (double versus single flowers) and flower colour (pink versus white), were mapped on linkage groups 3 and 2, respectively. The markers closest to the gene for double flowers, Blfo, and to the gene for pink flower colour, Blfa, cosegregated without recombinants. The maps provide a tool for further genetic analyses of horticulturally important genes as, for example, resistance genes and a starting point for marker-assisted breeding in roses.