The structure and immunoreactivity of exopolysaccharide isolated from Lactobacillus johnsonii strain 151
The structure and immunoreactivity of exopolysaccharide isolated from Lactobacillus johnsonii strain 151
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DOI：
10.1016/j.carres.2013.05.012
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发表时间：
2013-08-30
影响因子：
3.1
通讯作者：
Gamian, Andrzej
中科院分区：
文献类型：
--
作者：
Gorska-Fraczek, Sabina;Sandstrom, Corine;Gamian, Andrzej
对分离自小鼠肠道的约翰逊乳杆菌151菌株的胞外多糖(EPS)结构进行了研究。糖和甲基化分析结合H-1和C-13核磁共振波谱，包括二维H-1，H-1 COSY，TOCSY，NOESY和H-1，C-13 HSQC，HMBC实验表明，EPS的重复单元是线性五糖：->；6)-α-D-Galp-(1->；6)-α-D-Glcp-(1->；3)-β-D-Galf-(1->；3)-α-D-Glcp-(1-&g2)-β-D-Galf-(1->；从约翰逊乳杆菌(L.johnsonii)151和142(Carbohyr.)结果2010,345,108-114)，进行了比较。两种EPs与抗血清的反应性不同。约翰逊乳杆菌151的EPS与5个不同菌株的抗乳酸菌多克隆血清反应，而L.johnsonii 142的EPS仅与自身抗血清反应。151株的EPS比142株的EPS具有更广泛的特异性和更高的反应性。在成人和脐带血血清中均存在识别多糖抗原的生理抗体。从实验性炎症性肠病(IBD)小鼠中分离到一株高度特异的EPS 142菌株，而从健康小鼠肠道中分离到一株EPS 151菌株，该菌株具有广泛的免疫反应性共同结构。(C)2013爱思唯尔有限公司。保留所有权利。
The exopolysaccharide (EPS) structure from Lactobacillus johnsonii strain 151 isolated from the intestinal tract of mice was investigated. Sugar and methylation analyses together with H-1 and C-13 NMR spectroscopy, including two-dimensional H-1, H-1 COSY, TOCSY, NOESY, and H-1, C-13 HSQC, HMBC experiments, revealed that the repeating unit of the EPS is the linear pentasaccharide:-> 6)-alpha-D-Galp-(1 -> 6)-alpha-D-Glcp-(1 -> 3)-beta-D-Galf-(1 -> 3)-alpha-D-Glcp-(1 -> 2)-beta-D-Galf-(1 ->The immunoreactivity of two structurally different exopolysaccharides isolated from L. johnsonii, 151 and 142 (Carbohydr. Res. 2010, 345, 108-114), was compared. Both EPSs differed in their reactivity with antisera. EPS from L. johnsonii 151 reacted with anti-Lactobacillus polyclonal sera against cells of five different strains, while EPS from L. johnsonii 142 was found to react only with its own antiserum. The broader specificity and higher reactivity of EPS from 151 strain than EPS from 142 strain were also observed with human sera. The physiological antibodies recognizing polysaccharide antigens were present in both adults and umbilical cord blood sera. A highly specific EPS 142 bearing strain was isolated from experimentally induced inflammatory bowel disease (IBD) mice, while a strain with EPS 151 isolated from the intestinal tract of healthy mice is characterized by a broad immune reactivity common structure. (C) 2013 Elsevier Ltd. All rights reserved.