Comparision of Methods Used for Determination of Total Volatile Basic Nitrogen (TVB-N) in Rainbow Trout (Oncorhynchus mykiss)
Comparision of Methods Used for Determination of Total Volatile Basic Nitrogen (TVB-N) in Rainbow Trout (Oncorhynchus mykiss)
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发表时间：
2000-03
影响因子：
1
通讯作者：
F. Özoğul;Y. Özoğul
中科院分区：
文献类型：
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作者：
F. Özoğul;Y. Özoğul
以肌肉中挥发性盐基氮（TVB-N）含量为指标，比较了保鲜膜和铝箔包装虹鳟鱼的腐败程度。在4-6°C下，每两天测量一次不同肌肉组织和整条鱼中的TVB-N水平，为期16天。此外，三种不同的方法来确定TVB-N进行了评估的灵敏度，可靠性，速度和成本。无论分析方法如何，贮藏期间TVB-N水平都有所增加，尤其是在7-9天之后。总的来说，增加的趋势是相似的，在每种方法，但半水蒸气蒸馏产生的TVB-N的浓度最高。试验结果还表明，在两种方法中，腹肉样品的TVB-N值最高，而背肉样品的TVB-N值最低。在成本允许的情况下，建议采用流动注射法测定挥发性盐基氮。但同时也发现，挥发性盐基氮不能作为衡量样品腐败的指标，只能作为样品腐败程度的指示物。
TVB-N (total volatile basic nitrogen) concentration in fish muscle was used as an index of freshness quality to compare the spoilage of rainbow trout wrapped in cling film and in aluminium foil. TVB-N levels in different muscle tissue and in the whole fish were measured every two days for a period of 16 days at 4-6°C. In addition, three different methods of determining of TVB-N were evaluated in terms of sensitivity, reliability, speed, and cost. Regardless of the method of analysis, there was an increase in the level of TVB-N during storage, especially after 7-9 days. In general, the trend of increase was similar in each method, but semi-steam distillation yielded the highest concentration of TVB-N. Results obtained also showed that TVB-N in the belly flesh sample was the highest, whereas the lowest TVB-N value was obtained with the dorsal flesh for each method. If the high cost can be tolerated, flow injection method is recommended for determination of TVB-N. It was also found, however, that TVB-N can not be used as an index of spoilage but as an indicator of advanced spoilage in the sample.