A Multiplex RT-PCR Assay for Detection and Differentiation of Avian-Origin Canine H3N2, Equine-Origin H3N8, Human-Origin H3N2, and H1N1/2009 Canine Influenza Viruses.
A Multiplex RT-PCR Assay for Detection and Differentiation of Avian-Origin Canine H3N2, Equine-Origin H3N8, Human-Origin H3N2, and H1N1/2009 Canine Influenza Viruses.
复制标题
用于检测和区分禽源犬 H3N2、马源 H3N8、人源 H3N2 和 H1N1/2009 犬流感病毒的多重 RT-PCR 测定。
DOI：
10.1371/journal.pone.0170374
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Wang C;Wang Q;Hu J;Sun H;Pu J;Liu J;Sun Y
病毒学和血清学调查表明，H1N1/2009型流感病毒、禽源犬H3N2 （cH3N2）、季节性人源H3N2 （hH3N2）和马源H3N8型流感病毒在犬中持续传播。在本研究中，建立了一种多重逆转录酶聚合酶链反应（mRT-PCR）方法来同时检测和分化这些流感病毒。设计了4套引物，分别针对H1N1/2009、cH3N2、hH3N2和H3N8犬流感病毒（CIVs）的血凝素基因。该mRT-PCR检测对四种CIV亚型具有高特异性和敏感性。此外，从420份临床样本中获得的mRT-PCR结果与常规病毒分离方法获得的结果一致。我们的mRT-PCR检测是可靠的临床诊断和快速鉴定CIVs。
Virological and serological surveys have documented that H1N1/2009, avian-origin canine H3N2 (cH3N2), seasonal human-origin H3N2 (hH3N2), and equine-origin H3N8 influenza viruses are consistently circulating in dogs. In the present study, a multiplex reverse-transcriptase polymerase chain reaction (mRT-PCR) assay was developed for simultaneous detection and differentiation of these influenza viruses. Four primer sets were designed to target the hemagglutinin genes of H1N1/2009, cH3N2, hH3N2, and H3N8 canine influenza viruses (CIVs). This mRT-PCR assay demonstrated high specificity and sensitivity for the four CIV subtypes. Additionally, mRT-PCR results obtained from 420 clinical samples were consistent with those obtained by the conventional virus isolation method. Our mRT-PCR assay is reliable for clinical diagnosis and rapid identification of CIVs.