Novel subdomains of the mouse olfactory bulb defined by molecular heterogeneity in the nascent external plexiform and glomerular layers.
Novel subdomains of the mouse olfactory bulb defined by molecular heterogeneity in the nascent external plexiform and glomerular layers.
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DOI：
10.1186/1471-213x-7-48
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发表时间：
2007-05-16
影响因子：
--
通讯作者：
Lin DM
中科院分区：
文献类型：
--
作者：
Williams EO;Xiao Y;Sickles HM;Shafer P;Yona G;Yang JY;Lin DM
在小鼠嗅觉系统中，嗅球在引导嗅觉感觉神经元（OSN）轴突到达其靶点中的作用知之甚少。灯泡内的哪些细胞类型是靶向所必需的尚不清楚。哪些基因对这个过程很重要也是未知的。虽然投射神经元不是必需的，但在外部丛状和肾小球层内的其他细胞类型也与OSN形成突触。我们假设这些细胞对于靶向是重要的，并且表达空间差异表达的引导线索，其用于引导灯泡内的OSN轴突。我们使用激光显微切割和微阵列分析，以找到差异表达的基因，沿着背腹，内侧，和前后轴的灯泡。这些基因的表达模式将球茎分成以前未识别的子域。有趣的是，一些基因在内侧和外侧球中表达，首次显示了沿该轴沿着对称表达的存在。我们使用的再生范例表明，这些基因中的几个在响应于传入神经阻滞的表达中发生了改变，这与它们在与OSN相互作用的细胞中表达的解释一致。我们表明，新生的外部丛状和肾小球层的灯泡可以分为多个域的基础上，这些基因的表达，其中几个是已知的轴突导向，突触发生和血管生成的功能。这些基因代表候选的指导线索，可能会采取行动，以指导OSN轴突内的灯泡在靶向。
In the mouse olfactory system, the role of the olfactory bulb in guiding olfactory sensory neuron (OSN) axons to their targets is poorly understood. What cell types within the bulb are necessary for targeting is unknown. What genes are important for this process is also unknown. Although projection neurons are not required, other cell-types within the external plexiform and glomerular layers also form synapses with OSNs. We hypothesized that these cells are important for targeting, and express spatially differentially expressed guidance cues that act to guide OSN axons within the bulb. We used laser microdissection and microarray analysis to find genes that are differentially expressed along the dorsal-ventral, medial-lateral, and anterior-posterior axes of the bulb. The expression patterns of these genes divide the bulb into previously unrecognized subdomains. Interestingly, some genes are expressed in both the medial and lateral bulb, showing for the first time the existence of symmetric expression along this axis. We use a regeneration paradigm to show that several of these genes are altered in expression in response to deafferentation, consistent with the interpretation that they are expressed in cells that interact with OSNs. We demonstrate that the nascent external plexiform and glomerular layers of the bulb can be divided into multiple domains based on the expression of these genes, several of which are known to function in axon guidance, synaptogenesis, and angiogenesis. These genes represent candidate guidance cues that may act to guide OSN axons within the bulb during targeting.