THE RATE OF UPTAKE OF CARBON MONOXIDE AND OF NITRIC OXIDE BY NORMAL HUMAN ERYTHROCYTES AND EXPERIMENTALLY PRODUCED SPHEROCYTES
THE RATE OF UPTAKE OF CARBON MONOXIDE AND OF NITRIC OXIDE BY NORMAL HUMAN ERYTHROCYTES AND EXPERIMENTALLY PRODUCED SPHEROCYTES
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DOI：
10.1085/jgp.42.1.83
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发表时间：
1958-01-01
影响因子：
3.8
通讯作者：
COMROE, JH
中科院分区：
文献类型：
--
作者：
CARLSEN, E;COMROE, JH
红细胞对氧气、一氧化碳或一氧化氮等气体的吸收包括：（a）穿过细胞膜的扩散，（B）红细胞内扩散，以及（c）与血红蛋白的化学结合。这项调查的目的是获得数据，这将允许分析这些因素中的每一个限制气体吸收率。与血红蛋白化学结合联合收割机产生颜色变化的气体的初始总吸收速率可以通过改进的Hartridge-Roughton-Millikan恒流快速反应装置来测量。如果一氧化氮是反应气体，则仅测量（a）和（B），因为该气体与血红蛋白的化学结合极其迅速。我们的研究表明，人双凹面盘状红细胞在38和48[degree]C下具有与相同细胞转化为等体积球形红细胞相同的一氧化碳和一氧化氮摄取的初始速率。同样，椎间盘和海绵状细胞之间没有差异，体积增加了30%。收缩的红细胞显示出气体摄取速率的显著降低。这表明，该量级的细胞内扩散的表面积和最大线性距离不会显著延迟气体摄取。在收缩的细胞中，红细胞内血红蛋白和/或膜成分的取向和浓度的变化可能阻碍了气体扩散。
The uptake of gases such as oxygen, carbon monoxide, or nitric oxide by the erythrocyte involves: (a) diffusion across the cellular membrane, (b) intraerythrocytic diffusion, and (c) chemical combination with hemoglobin. The aim of this investigation was to obtain data which would permit an analysis of each of these factors in limiting the rate of gas uptake. The initial over-all rate of uptake of gases which combine chemically with hemoglobin to produce a color change can be measured by a modified version of the Hartridge-Roughton-Millikan constant flow, rapid reaction apparatus. If nitric oxide is the reactant gas, only (a) and (b) are measured since the chemical combination of this gas with hemoglobin is extremely rapid. Our studies have shown that human biconcave discoidal erythrocytes at 38 and 48[degree]C, have the same initial rate of carbon monoxide and nitric oxide uptake as the same cells converted into spherocytes of equal volume. Similarly there was no difference between discs and cells sphered with a 30 per cent increase in volume. Shrunken erythrocytes showed a marked decrease in rate of gas uptake. This suggests that surface area and maximum linear distance for intracellular diffusion of this magnitude do not measurably retard gas uptake. In the shrunken cells, a change in the orientation and concentration of intraerythrocytic hemoglobin and/or of the membrane components may have impeded gas diffusion.