DEVELOPMENT OF HISTAMINE-IMMUNOREACTIVE NEURONS IN THE RAT-BRAIN
DEVELOPMENT OF HISTAMINE-IMMUNOREACTIVE NEURONS IN THE RAT-BRAIN
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DOI：
10.1002/cne.902760211
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发表时间：
1988-10-08
影响因子：
2.5
通讯作者：
PANULA, P
中科院分区：
文献类型：
--
作者：
AUVINEN, S;PANULA, P
本研究旨在揭示发育中的大鼠脑中组胺的细胞储存，并确定组胺免疫反应神经元最早可被检测到的发育阶段。从胚胎第12天至出生后第14天对大鼠进行研究。脑用4% 1-乙基-3（3-二甲氨基丙基）碳二亚胺固定，采用标准免疫荧光技术。第一个组胺免疫反应神经元在胚胎第13天出现在中脑和后脑交界处。胚胎日在腹侧中脑和菱形脑检测到15个免疫反应神经元。在胚胎第20天，尾侧、结节和乳后尾侧大细胞核中首次检测到组胺免疫反应神经元，而后脑中的组胺免疫反应神经元已消失。组胺免疫反应性神经纤维在胚胎第15天首次出现在左脑和中脑以及间脑的部分区域，包括乳状体和额叶皮层。胚胎第18天，后脑免疫反应神经纤维数量明显减少，但嗅球、中隔、下丘脑区和大脑皮层出现免疫反应。组胺免疫反应纤维网络的密度增加，直到出生后第14天，神经元和纤维已经发育成成人的模式。组胺免疫反应神经元存在于胚胎中枢神经系统中，它们向大脑各区域发展广泛的投射。
This study was undertaken to reveal the cellular stores of histamine in developing rat brain and to determine the stage of development during which the histamine-immunoreactive neurons can first be detected. Rats from embryonal day 12 to postnatal day 14 were studied. The brains were fixed in 4% 1-ethyl-3(3-dimethylaminopropyl)carbodiimide and standard immunofluorescence technique was used. The first histamine-immunoreactive neurons were seen on embryonic day 13 in the border of mesencephalon and metencephalon. On embryonic day 15 immunoreactive neurons were detected in ventral mesencephalon and rhombencephalon. In caudal, tuberal, and postmammillary caudal magnocellular nuclei histamine-immunoreactive neurons were first detected on embryonic day 20 while those in the hindbrain had disappeared. Histamine-immunoreactive nerve fibers were first detected on embryonic day 15 in rhombencephalon and mesencephalon and in some areas of diencephalon including the mammillary bodies and frontal cortex. On embryonic day 18 the number of immunoreactive nerve fibers in the hindbrain had decreased considerably, but the olfactory bulb, septal and hypothalamic area, and the cerebral cortex showed immunoreaction in fibers. The density of histamine-immunoreactive fiber networks increased until postnatal day 14 when an adultlike pattern of neurons and fibers had developed. Histamine-immunoreactive neurons are present in embryonal CNS and they develop extensive projections to various brain areas.