<b>Genetic characteristics of </b><b><i>Pyricularia grisea</i></b><b> </b><b>and their pathotypes in Myanmar </b>

<b>Genetic characteristics of </b><b><i>Pyricularia grisea</i></b><b> </b><b>and their pathotypes in Myanmar </b>
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<b>缅甸稻瘟病菌的遗传特征及其致病型</b><b><i>稻瘟病菌</i></b><b> </b><b>

DOI:
10.11189/bita.39.103
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发表时间:
2016
期刊:
Bulletin of the Institute of Tropical Agriculture, Kyushu University
影响因子:
--
通讯作者:
M. Matsumoto
M. Matsumoto
中科院分区:
--
文献类型:
--
作者:
M. Zaw;S.S. Aye;M. Matsumoto

文献摘要

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稻瘟病是缅甸水稻的主要破坏性真菌病害之一。 2011年,从缅甸收集到46株稻瘟病菌。分别使用 Pot2-TIR 和 MGR586-TIR 引物通过 Rep-PCR 确定稻瘟病菌的种群结构。根据不同的指纹模式构建组合树状图。确定了三个指纹组(指定为 A 型、B 型和 C 型)。 A 型和 B 型主要存在于 Aung Ban 地区(掸邦南部),而 C 型则主要存在于 Hmawbi 地区(仰光省)。通过接种一组 12 个日本差异水稻品种来检查每个指纹组的代表性分离株的致病型,并鉴定出 9 个小种(031.0、032.0、036.0、106.4、122.4、132.4、136.4、432.5 和 437.5)。这是缅甸 P. girsea 遗传和致病型变异的首次表征,遗传指纹图谱与致病型种族之间没有明确的关联。
Rice blast disease is one of the major destructive fungal diseases of rice in Myanmar. In 2011, forty-six isolates of Pyricularia grisea were collected from Myanmar. The population structure of the P. grisea was determined by Rep-PCR using Pot2-TIR and MGR586-TIR primers separately. The combined dendrogram was constructed based on the different fingerprint patterns. Three fingerprint groups (designated as Type-A, Type-B and Type-C) were identified. Type-A and B were dominantly existed in Aung Ban area (Southern Shan State) whereas Type-C was prevalence in Hmawbi area (Yangon Division). Pathotypes of the representative isolates from each fingerprint group were examined by inoculating on a set of twelve Japanese differential rice cultivars and nine races (031.0, 032.0, 036.0, 106.4, 122.4, 132.4, 136.4, 432.5 and 437.5) were identified. This is the first characterization of genetic and pathotypic variations of P. girsea in Myanmar and there was no clear association between the genetic fingerprint profiles and pathotypic races.