Mercury bioremediation in aquatic environment by genetically modified bacteria with self-controlled biosecurity circuit
Mercury bioremediation in aquatic environment by genetically modified bacteria with self-controlled biosecurity circuit
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利用自控生物安全回路的转基因细菌对水生环境中的汞进行生物修复
DOI：
10.1016/j.jclepro.2022.130524
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发表时间：
2022-01
影响因子：
11.1
通讯作者：
Yu Bo
中科院分区：
文献类型：
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作者：
Xue Yubin;Du Pei;Amal Amin Ibrahim Shendi;Yu Bo
重金属污染如汞（Hg 2+）通过食物链富集并最终进入人体，对全球粮食安全构成严重威胁。生物修复方法是有前途的，但在某些情况下，天然微生物并不理想的实际应用，这需要对这些生物进行遗传修饰。然而，转基因细菌被释放到环境中的威胁长期以来一直是限制这种技术应用的主要问题。在这项研究中，我们设计并优化了一个基因电路，能够激活Hg 2+吸附模块后，传感器Hg 2+在水体中，并杀死细胞自杀模块以可编程的方式。利用该电路，工程化大肠杆菌细胞仅在Hg ~（2+）浓度超过一定阈值时才表达Hg ~（2+）吸附蛋白。然后，吸附有Hg 2+的细胞可以从自然环境中去除与磁固定化策略和剩余的细胞被编程杀死时，Hg 2+浓度下降到低于阈值的水体。重要的是，自杀模块经过精心优化，以确保逃逸率低于10−9，这符合美国NIH指南的建议。吸附池可以重复使用5次，Hg 2+吸附效率稳定在95%以上，逃逸率低于10−9。因此，这项研究的进展为在开放环境中直接使用工程微生物提供了线索。
Heavy metal pollution such as mercury (Hg2+) poses a severe threat to food security worldwide because of enrichment through food chain and, eventually, to the human body. Biological remediation approaches are promising, but in some cases, the natural microorganisms are not ideal for practical application, which requires genetic modification of such organisms. However, the threat of genetically modified bacteria being released into the environments has long been the major concern that limits the applications of such technologies. In this study, we designed and optimized a genetic circuit that is capable of activating a Hg2+adsorption module after sensing Hg2+in waterbody, and killing cells with a cell suicide module in a programmable manner. With this circuit, the engineeredEscherichia colicells are programmed to express Hg2+adsorption protein only when Hg2+concentration is above a certain threshold. Then, cells absorbed with Hg2+can be removed from natural environments with magnetically immobilized strategy and the remaining cells are programmed to be killed by the suicide module when Hg2+concentration drops below a threshold in waterbody. Importantly, the suicide module was carefully optimized to ensure the escape rate is below 10−9, which meets the recommendation demanded by U.S. NIH guideline. The absorption cells could be reused for 5 cycles, with an Hg2+adsorption efficiency steadily above 95% and escape rates below 10−9. Thus, the advancement of this study sheds light on using engineered microbes directly in an open circumstance.
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影响因子：
4.7
作者：
St-Pierre, Francois;Cui, Lun;Shearwin, Keith E.
通讯作者：
Shearwin, Keith E.
影响因子：
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影响因子：
64.8
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