Expression of αv and β3 integrin subunits in rat osteoclasts in situ
Expression of αv and β3 integrin subunits in rat osteoclasts in situ
复制标题
αv和β3整合素亚基在大鼠破骨细胞中的原位表达
DOI：
10.1002/jbmr.5650080404
复制
发表时间：
1993
影响因子：
6.2
通讯作者：
M. Weinreb
中科院分区：
文献类型：
--
作者：
D. Shinar;A. Schmidt;D. Halperin;G. Rodan;M. Weinreb
最近的研究表明，作为整合素家族的一员，玻璃连结蛋白受体在破骨细胞与骨基质的附着中起着重要的作用。本文报道用原位杂交技术对大鼠骨切片中玻璃体连接素受体α和β亚单位的基因进行定位。为了制备本研究中所用的大鼠特异性RNA探针，我们通过聚合酶链式反应从大鼠中克隆了整合素链αv、β3和β5的c DNA片段。这些片段与相应的人类序列有86-91%的同源性。原位杂交将αv和β3mRNAs定位于发生广泛骨吸收的区域。组织学观察和抗酒石酸酸性磷酸酶(TRAP)染色结果表明，破骨细胞中存在这些基因。这些观察结果支持破骨细胞高水平表达玻璃体连接素受体的免疫组织化学结果，证实了该受体为整合素αv/β3，并提示破骨细胞可能主动合成这些分子。
Recent findings suggest that the vitronectin receptor, a member of the integrin family, plays an important role in the attachment of osteoclasts to bone matrix. We report here the localization by in situ hybridization of the mRNA for the α and β subunits of the vitronectin receptor in rat bone sections. To generate the rat‐specific RNA probes used in this study, we cloned cDNA fragments of integrin chains αv, β3, and β5 by the polymerase chain reaction from rat cDNA. These fragments share 86–91% homology with the respective human sequences. In situ hybridization localized the αv and β3 mRNAs to regions undergoing extensive bone resorption. The histologic appearance and prestaining of bone sections for tartrate‐resistant acid phosphatase (TRAP) indicated the presence of these mRNAs in osteoclasts. These observations support immunohis‐tochemical findings that osteoclasts express high levels of the vitronectin receptor, confirm the identity of this receptor as integrin αv/β3, and suggest that osteoclasts may actively synthesize these molecules.