High Incidence of Non-B and Recombinant HIV-1 Strains in Newly Diagnosed Patients in Galicia, Spain: Study of Genotypic Resistance
High Incidence of Non-B and Recombinant HIV-1 Strains in Newly Diagnosed Patients in Galicia, Spain: Study of Genotypic Resistance
复制标题
西班牙加利西亚新诊断患者中非 B 型和重组 HIV-1 毒株的高发病率：基因型耐药性研究
DOI：
10.1177/135965350300800413
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发表时间：
2002
影响因子：
1.2
通讯作者：
J. Torres
中科院分区：
文献类型：
--
作者：
L. Pérez;Rocío Carmona;Mercedes Muñoz;E. Delgado;M. Thomson;G. Contreras;J. Pedreira;R. Real;E. V. de Parga;L. Medrano;J. Taboada;R. Nájera;A. Agulla;A. Mariño;S. López;J. Pedreira;A. Aguilera;E. Losada;A. Prieto;J. Corredoira;M. López Álvarez;A. Rodríguez;M. Bustillo;J. García;R. Fernández;R. Rodríguez;C. Miralles;A. Ocampo;R. Rodríguez;J. Diz;R. O. de Castro;L. Morano;R. Perez;A. Rodríguez;J. Torres
本研究的目的是描述2年期间西班牙西北部加利西亚地区新诊断的HIV-1感染中非B和重组HIV-1毒株的发病率（2000年5月至2002年6月），以及逆转录酶（RT）和蛋白酶（PR）基因中耐药相关突变的频率，分析非B和重组病毒中更常见的多态性。在加利西亚七个主要城市的九家公立医院就诊的所有新诊断的HIV-1感染者都被纳入这项研究。对HIV-1 RNA血浆的RT、PR和V3区进行扩增和测序，将校正后的序列发送到斯坦福大学HIV RT和蛋白酶序列数据库。85例患者中有19例（22.3%）被非B或重组病毒感染：3例亚型C，2例G，1例F1，1例Dpol/A1 V3，5例CRF02_AG，1例CRF14_BG，5例BGpol/BV 3和1例UKpol/UV 3（U，未知片段）。这19名患者中有11人（57.9%）是居住在加利西亚的外国人，通过异性接触感染，另外8人（42.1%）是西班牙静脉注射吸毒者，他们共用注射设备。85例患者中有5例（5.9%）显示RT耐药相关突变（M184 V、M41 L、L210 W、T215 Y/D和K219 Q），均感染B亚型病毒。在1例G亚型病毒感染患者（1.2%）中，检测到PR耐药相关突变（K20 I + M36 I + M46 I + V82 I）。在B亚型病毒中未检测到PR的耐药突变。RT中的几种多态性：D123 S、Q174 K、D177 E、T200 A、V245 Q和PR：I13 V、K20 I、M36 I、R41 K、H69 K、L 89 M在非B和重组菌株中的检出率高于B菌株（P<0.01 ~ P<0.001）。这项研究报告了高发病率（22.3%）的新诊断的患者感染不同的非B和重组HIV-1株，在西班牙的地理区域，也表现出高频率的RT和PR基因的多态性。
The objectives of this study are to describe the incidence of non-B and recombinant HIV-1 strains in newly diagnosed HIV-1 infections in Galicia, northwest of Spain, during a 2-year period (May 2000 to June 2002), and the frequency of resistance-associated mutations in reverse transcriptase (RT) and protease (PR) genes, analysing the polymorphisms more frequently detected in non-B and recombinant viruses. All newly diagnosed HIV-1-infected patients attending the nine public hospitals of the seven main cities of Galicia were included in this study. RT, PR and V3 regions from HIV-1 RNA plasma were amplified and sequenced, being the corrected sequences sent to the Stanford HIV RT and Protease Sequence Database. Nineteen of 85 patients (22.3%) were infected by non-B or recombinant viruses: three subtype C, two G, one F1, one Dpol/A1V3, five CRF02_AG, one CRF14_BG, five BGpol/BV3 and one UKpol/UV3 (U, unknown fragment). Eleven of these 19 patients (57.9%) were foreign individuals living in Galicia infected through heterosexual contact, and the other eight (42.1%) were Spanish intravenous drug users who had shared injection equipment. Five of 85 patients (5.9%), all infected with B subtype viruses, showed resistance-associated mutations in RT (M184V, M41L, L210W, T215Y/D and K219Q). In one patient (1.2%) infected with a subtype G strain, resistance-associated mutations in PR (K20I+M36I+M46I+V82I) were detected. In subtype B viruses resistance mutations in PR were not detected. Several polymorphisms in RT: D123S, Q174K, D177E, T200A, V245Q, and PR: I13V, K20I, M36I, R41K, H69K, L89M were detected more frequently in non-B and recombinants than in B strains (P<0.01 to P<0.001). This study reports a high incidence (22.3%) of newly diagnosed patients infected by different non-B and recombinant HIV-1 strains, in a geographical area of Spain, showing also a high frequency of polymorphisms in RT and PR genes.
影响因子：
120.7
作者：
Grant, RM;Hecht, FM;Kahn, JO
通讯作者：
Kahn, JO
影响因子：
158.5
作者：
Little, SJ;Holte, S;Richman, DD
通讯作者：
Richman, DD
影响因子：
120.7
作者：
Boden, D;Hurley, A;Markowitz, M
通讯作者：
Markowitz, M