FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
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DOI：
10.1016/j.diagmicrobio.2019.114880
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发表时间：
2019-12-01
影响因子：
2.9
通讯作者：
Stefanovic, Aleksandra
中科院分区：
文献类型：
--
作者：
Locher, Kerstin;Roscoe, Diane;Stefanovic, Aleksandra
BioFire薄膜阵列呼吸板(FA RP)是一种快速多重分子检测方法，被批准用于检测鼻咽标本中的病毒和非典型细菌病原体。本研究旨在评估BioFire FilmArray呼吸面板v1.7在支气管镜标本上的性能。我们用FA RP检测了133例支气管标本(87例存档，46例预期收集)，并将结果与Luminex NxTAG呼吸道病原体专家组(NxTAG RPP)进行了比较。经不一致分析，123例标本FA-RP与NxTAG-RPP的总符合率为93.9%(kappa=0.88[95%CI为0.80~0.96])，阳性符合率为93.7%(95%CI为83.7~97.7)，阴性符合率为94.1%(95%CI为84.9~98.1)。总之，BioFire FilmArray RP在检测支气管镜标本中的广泛呼吸道病原体方面表现可靠。(C)2019 Elsevier Inc.保留所有权利。
The BioFire FilmArray Respiratory Panel (FA RP) is a rapid multiplexed molecular assay approved for detection of viral and atypical bacterial pathogens in nasopharyngeal specimens. This study aimed to evaluate the performance of the BioFire FilmArray Respiratory Panel v1.7 on bronchoscopy specimens. We tested 133 bronchial specimens (87 archived and 46 prospectively collected) with the FA RP and compared the results to the Luminex NxTAG Respiratory Pathogen Panel (NxTAG RPP). After discordant analysis, 123 specimens gave concordant results using the FA RP and the NxTAG RPP for an overall agreement of 93.9% (kappa = 0.88 [95% CI 0.80-0.96]), a positive percent agreement of 93.7% (95% CI 83.7-97.7) and a negative percent agreement of 94.1% (95% CI 84.9-98.1). In conclusion, the BioFire FilmArray RP performed reliably to detect a broad range of respiratory pathogens in bronchoscopy specimens. (C) 2019 Elsevier Inc. All rights reserved.