A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice
A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice
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DOI：
10.1016/j.foodchem.2020.127710
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发表时间：
2021-01-30
期刊：
影响因子：
8.8
通讯作者：
Xiong, Yonghua
中科院分区：
文献类型：
--
作者：
Hao, Liangwen;Chen, Jing;Xiong, Yonghua
传统的基于纳米金的侧流免疫分析法（LFIA）在测定深色食品基质中的目标浓度时，由于其对背景基质和颜色干扰的抵抗力差，面临着巨大的挑战。为了解决这个问题，我们首先报告了一种新的双功能磁-金纳米杂化物（MGNH），通过将MGNH集成到LFIA中，用于同时进行磁分离和比色目标检测。在最佳条件下，葡萄汁中赭曲霉毒素A（OTA）的检出限为0.094 ng mL（-1）。该方法的平均回收率为92.31%~ 108.97%，变异系数小于12%。结果表明，MGNH-LFIA对OTA具有良好的选择性。此外，我们的MGNH-LFIA在准确性、重现性和实用性方面与液相色谱-质谱联用相当。所设计的MGNH-LFIA平台很容易扩展，用于改善食品样品中其他小分子的检测。
Conventional gold nanoparticle-based lateral flow immunoassay (LFIA) usually suffers a huge challenge in measuring target concentration in food matrices with dark color because of its poor resistance to the background matrix and color interference. To address this issue, we first report a novel bifunctional magneto-gold nanohybrid (MGNH) for the simultaneous magnetic separation and colorimetric target sensing by integrating MGNHs into LFIA. Under optimum conditions, an ultrasensitive detection of ochratoxin A (OTA) in grape juice was achieved with a limit of detection at 0.094 ng mL(-1). The average recoveries of this MGNH-LFIA ranged from 92.31% to 108.97% with a coefficient of variation of below 12%. The excellent selectivity of our MGNH-LFIA against OTA was demonstrated. Besides, our MGNH-LFIA is comparable to liquid chromatography coupled with mass spectrometry in terms of accuracy, reproducibility, and practicability. The designed MGNH-LFIA platform is readily extended for improving other small molecule detection in food samples.