Phlorotannins from Brown Algae (Fucus vesiculosus) Inhibited the Formation of Advanced Glycation Endproducts by Scavenging Reactive Carbonyls
Phlorotannins from Brown Algae (Fucus vesiculosus) Inhibited the Formation of Advanced Glycation Endproducts by Scavenging Reactive Carbonyls
复制标题
DOI：
10.1021/jf204112f
复制
发表时间：
2012-02-08
影响因子：
6.1
通讯作者：
Gu, Liwei
中科院分区：
文献类型：
--
作者：
Liu, Haiyan;Gu, Liwei
晚期糖基化终产物（AGEs）在体内的积累与衰老、糖尿病、阿尔茨海默病、肾功能衰竭等有关。本研究的目的是探讨褐藻墨角藻根皮单宁对AGEs形成的抑制作用。F.用70%丙酮提取囊泡状根皮单宁。将所得萃取物分馏成二氯甲烷、乙酸乙酯、丁醇和水馏分。使用Sephadex LH-20柱将乙酸乙酯级分进一步分馏成四个亚级分（乙基-F1至-F4）。F.结果表明，囊泡酵母丙酮提取物或组分以浓度依赖性方式显著抑制葡萄糖和丙酮醛介导的AGEs的形成。F.在牛血清白蛋白（BSA）-甲基乙二醛试验中，囊泡提取物抑制50%白蛋白糖基化（EC 50）的要求低于氨基胍（一种治疗糖尿病并发症的候选药物）。囊泡菌丙酮提取物和二氯甲烷级分。在BSA-葡萄糖测定中，F.除了乙基-F3和-F4外，囊泡提取物抑制BSA糖基化的效果比氨基胍更好或与氨基胍一样有效。乙酸乙酯馏分及其4个亚馏分在2小时内对丙酮醛的清除率均在50%以上。假设F.测试了囊泡状根皮单宁通过形成加合物清除活性羰基。间苯三酚是间苯单宁的组成单元，可与乙二醛和甲基乙二醛反应。利用HPLC-ESI-MSn检测并初步鉴定了5种间苯三酚羰基加合物。
Accumulation of advanced glycation end products (AGEs) in vivo is associated with aging, diabetes, Alzheimer's disease, renal failure, etc. The objective of this study was to investigate the inhibitory effects of brown algae Fucus vesiculosus phlorotannins on the formation of AGEs. F. vesiculosus phlorotannins were extracted using 70% acetone. The resultant extract was fractionated into dichloromethane, ethyl acetate, butanol, and water fractions. The ethyl acetate fraction was further fractionated into four subfractions (Ethyl-F1 to -F4) using a Sephadex LH-20 column. F. vesiculosus acetone extract or fractions significantly inhibited the formation of AGEs mediated by glucose and methylglyoxal in a concentration-dependent manner. The concentrations of F. vesiculosus extracts required to inhibit 50% of albumin glycation (EC50) in the bovine serum albumin (BSA)-methylglyoxal assay were lower than those of aminoguanidine (a drug candidate for diabetic complication), except for F. vesiculosus acetone extract and dichloromethane fraction. In the BSA-glucose assay, F. vesiculosus extracts inhibited BSA glycation more than or as effectively as aminoguanidine, except for Ethyl-F3 and -F4. The ethyl acetate fraction and its four subfractions scavenged more than 50% of methylglyoxal in two hours. The hypothesis whether F. vesiculosus phlorotannins scavenged reactive carbonyls by forming adducts was tested. Phloroglucinol, the constituent unit of phlorotannins, reacted with glyoxal and methylglyoxal. Five phloroglucinol-carbonyl adducts were detected and tentatively identified using HPLC-ESI-MSn.