Production of a monoclonal antibody specific for seminomas and dysgerminomas.
Production of a monoclonal antibody specific for seminomas and dysgerminomas.
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生产精原细胞瘤和无性细胞瘤特异性单克隆抗体。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
A. Marks
中科院分区：
文献类型：
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作者：
D. Bailey;R. Baumal;J. Law;K. Sheldon;P. Kannampuzha;M. Stratis;H. Kahn;A. Marks
制备了抗培养的人卵巢上皮性腺癌细胞系HEY的单克隆抗体（M2 A，IgG 2a）。单克隆抗体M2 A与HEY细胞表面分子量为40，000的糖蛋白反应。单克隆抗体M2 A对HEY细胞的亲和常数为10（9）M-1，HEY细胞上的结合位点数为每个细胞2 × 10（4）。单克隆抗体产生阳性免疫过氧化物酶染色的胎儿（而不是成人）睾丸和卵巢癌和无性细胞瘤，但不染色各种正常成人组织或其他性腺或性腺外肿瘤。单克隆抗体M2 A可能有助于确认乳腺癌和无性细胞瘤的组织学诊断。
A monoclonal antibody (M2A, IgG2a) was produced against a cultured human ovarian epithelial adenocarcinoma cell line, HEY. Monoclonal antibody M2A reacted with a glycoprotein of molecular weight 40,000 on the surface of HEY cells. The affinity constant of the monoclonal antibody M2A for HEY cells was 10(9) M-1, and the number of binding sites on HEY cells was 2 X 10(4) per cell. The monoclonal antibody produced positive immunoperoxidase staining of fetal (but not adult) testis and of seminomas and dysgerminomas but did not stain various normal adult tissues or other gonadal or extragonadal tumors. Monoclonal antibody M2A may be useful for confirming a histological diagnosis of seminoma and dysgerminoma.