Resveratrol potentiates intracellular ascorbic acid enrichment through dehydroascorbic acid transport and/or its intracellular reduction in HaCaT cells.
Resveratrol potentiates intracellular ascorbic acid enrichment through dehydroascorbic acid transport and/or its intracellular reduction in HaCaT cells.
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白藜芦醇通过 HaCaT 细胞中的脱氢抗坏血酸转运和/或其细胞内减少来增强细胞内抗坏血酸富集。
DOI：
10.1007/s11010-020-03700-2
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Nakawa A
中科院分区：
文献类型：
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作者：
Saitoh Y;Umezaki T;Yonekura N;Nakawa A
l-抗坏血酸（AsA）是一种还原性维生素C (VC)，是一种重要的抗氧化剂，AsA的体内积累和维持被认为在人体各种生理活动中起着重要作用。我们研究了白藜芦醇（RSV），一种天然多酚化合物，作为AsA转运调节剂的候选物，并研究了在HaCaT角质形成细胞中添加AsA或脱氢抗坏血酸（DHA）后，RSV是否会影响细胞内VC的积累。我们的研究结果表明，RSV处理可以显著提高AsA或DHA补充后的细胞内VC水平，并且细胞内VC主要以AsA的形式积累。我们的研究结果还表明，大部分细胞内运输的DHA在进入细胞后被还原为AsA并积累。此外，RSV还能诱导多种AsA或DHA转运相关基因和细胞内DHA还原相关基因，包括svct2、GLUT3、TXNRD2和txnrd3，这些基因分别是AsA转运、DHA转运和DHA还原/再生所必需的。另一方面，钠依赖性维生素C转运体2 （SVCT2）和葡萄糖转运体3（GLUT3）的蛋白表达水平和定位几乎不受RSV处理的影响。此外，RSV诱导的细胞内AsA水平的富集被GLUT抑制剂细胞松弛素b完全抑制。这些结果表明，RSV可以通过激活DHA运输和随后从DHA到AsA的细胞内还原来增强细胞内AsA的积累。因此，RSV可能有助于维持皮肤角质形成细胞中AsA的大量积累。
l-Ascorbic acid (AsA), a reduced vitamin C (VC), is an important antioxidant, and the internal accumulation and maintenance of AsA are thought to play a significant role in various physiological activities in humans. We focused on resveratrol (RSV), a natural polyphenolic compound, as a candidate for an AsA transport modulator and investigated whether RSV can affect the intracellular VC accumulation after either AsA or dehydroascorbic acid (DHA) addition in HaCaT keratinocytes. Our results demonstrate that RSV treatment could significantly enhance intracellular VC levels after either AsA or DHA supplementation, and intracellular VC accumulated mainly as AsA. Our results also indicate that most of the intracellular transported DHA was reduced to AsA and accumulated after uptake into cells. In addition, RSV could induce several AsA or DHA transport-related and intracellular DHA reduction-related genes includingSVCT2,GLUT3,TXNRD2, andTXNRD3, necessary for AsA transport, DHA transport, and DHA reduction/regeneration, respectively. On the other hand, the both protein expression levels and the localizations of sodium-dependent vitamin C transporters 2 (SVCT2) and glucose transporter 3(GLUT3) were scarcely affected by RSV treatment. Furthermore, RSV-induced enrichment of intracellular AsA levels was completely suppressed by a GLUT inhibitor cytochalasin B. These results suggest that RSV can potentiate intracellular AsA accumulation via activation of the DHA transport and subsequent intracellular reduction from DHA to AsA. Thus, RSV might be useful for maintaining substantial AsA accumulation in the skin keratinocytes.