Looking beyond morphology: Cancer gene expression profiling using DNA microarrays
Looking beyond morphology: Cancer gene expression profiling using DNA microarrays
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DOI：
10.1081/cnv-120025096
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Duggan, DJ
中科院分区：
文献类型：
--
作者：
Luo, J;Isaacs, WB;Duggan, DJ
尽管分子检测已越来越多地应用于临床实践，但大多数人类癌症的精确诊断和预后仍然严重依赖描述性组织病理学数据。鉴定与独特形态参数相关的稳健分子标记将有助于诊断和预后评估。另一方面，形态上相同/相似的癌症的多样性可以在多个层面上表现出来，最重要的是在临床结果层面上。在过去几年中，DNA 微阵列技术已广泛应用于癌症研究领域，产生了大量新信息，可用于识别所有肿瘤共有的分子改变以及癌症亚类特有的特征谱。这些新发现很可能在短期内改变癌症患者的临床管理。本文回顾了应用 DNA 微阵列技术得出的癌症基因表达谱结果的最新进展，重点是对人类前列腺癌样本进行的研究。我们讨论与癌症表达谱相关的广泛问题，并尝试在适当的情况下使用前列腺癌作为例子来说明新发现的快速发展。
Although molecular testing has been increasingly used in clinical practice, the precise diagnosis and prognosis of most human cancers still heavily rely on descriptive histopathological data. Identification of robust molecular markers associated with distinctive morphological parameters will assist in diagnostic and prognostic assessment. On the other hand, the diversity of morphologically identical/similar cancers can be manifested at multiple levels, most importantly at the level of clinical outcome. In the past several years, DNA microarray technology has been widely used in the context of cancer research, resulting in a deluge of new information that can be used to identify molecular alterations common to all tumors as well as signatory profiles unique to a subcategory of cancers. These new findings will very likely transform the clinical management of cancer patients in the near term. This article reviews recent advances in cancer gene expression-profiling results derived from the application of DNA microarray technology, with an emphasis on studies performed on human prostate cancer specimens. We discuss broad issues relevant to cancer expression profiling and attempt to illustrate the rapid pace of novel discoveries using prostate cancer as examples wherever appropriate.