Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital.
Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital.
复制标题
三级护理医院尿液样本中分离出的 CTX-M-14 和 CTX-M-15 大肠杆菌和肺炎克雷伯菌的特征。
DOI：
10.4014/jmb.1306.06036
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发表时间：
2014
影响因子：
2.8
通讯作者：
S. Koo
中科院分区：
文献类型：
--
作者：
Semi Kim;J. Sung;Hye Hyun Cho;K. Kwon;S. Koo
本研究的目的是了解尿路分离的大肠埃希菌和肺炎克雷伯菌的CTX-M产生菌的特征，并确定它们中质粒介导的耐药基因的流行情况。测定最低抑菌浓度(MIC)，并进行聚合酶链式反应和测序。在42株(82.3%)肺炎克雷伯菌和24株(77.4%)肺炎克雷伯菌中，分别检出含有blA(CTX-M)、blA(CTX-M-14)和blA(CTX-M-15)的大肠埃希菌23株和19株，肺炎克雷伯菌7株和17株。产CTX-M尿液中的大肠埃希菌和肺炎克雷伯菌对多种抗生素耐药，并含有其他耐药基因。CTX-M-15菌株比CTX-M-14菌株含有更多的耐药基因。
This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and 24 (77.4%) K. pneumoniae isolates containing bla(CTX-M), bla(CTX-M-14) and bla(CTX-M-15) were detected in 23 and 19 E. coli isolates, respectively, and in 7 and 17 K. pneumoniae isolates, respectively. CTX-M producers of urinary E. coli and K. pneumoniae were resistant to multiple antibiotics and contained other antimicrobial resistance genes. CTX-M-15 producers contained more antimicrobial resistance genes than did CTX-M-14 producers.