Structural studies of Vgamma2Vdelta2 T cell phosphoantigens.
Structural studies of Vgamma2Vdelta2 T cell phosphoantigens.
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Vgamma2Vdelta2 T 细胞磷酸抗原的结构研究。
DOI：
10.1016/j.chembiol.2006.08.007
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发表时间：
2006
影响因子：
--
通讯作者：
Oldfield,Eric
中科院分区：
文献类型：
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作者：
Zhang,Yonghui;Song,Yongcheng;Yin,Fenglin;Broderick,Erin;Siegel,Kathryn;Goddard,Amanda;Nieves,Edward;Pasa-Tolic,Ljiljana;Tanaka,Yoshimasa;Wang,Hong;Morita,CraigT;Oldfield,Eric
Human γδ T cells containing the Vγ2Vδ2 (Vγ9Vδ2) T cell receptor are stimulated by a broad variety of small, phosphorus-containing antigenic molecules called phosphoantigens. The structures of several species present in both Mycobacteria (TUBags1–4) and inEscherichia colihave been reported to contain a formyl-alkyl diphosphate core. Here we report the synthesis of the lead member of the series, 3-formyl-1-butyl diphosphate. This compound has low activity for γδ T cell stimulation, unlike its highly active isomer (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate, necessitating a revision of the structure of TUBag1. Likewise, the structure of the species identified as the pentyl analog (TUBag 2) is revised to 6-phosphogluconate. These results indicate that neither TUBag1 nor the m/e 275 species proposed for TUBag2 are 3-formyl-1-alkyl diphosphates, leading to the conclusion that none of the natural phosphoantigens (TUBags1–4) possess the structures reported previously.
