An SPR based sensor for allergens detection
An SPR based sensor for allergens detection
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DOI：
10.1016/j.bios.2016.07.101
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发表时间：
2017-02-15
影响因子：
12.6
通讯作者：
Tothill, I. E.
中科院分区：
文献类型：
--
作者：
Ashley, J.;Piekarska, M.;Tothill, I. E.
以酪蛋白为生物标志物检测牛奶中的过敏原，建立了一种简单、灵敏、无标记的光学传感器分析变应原的方法，可直接用于食品制造商现场清洗系统(CIP)的最终漂洗样品。本工作采用表面等离子体共振(SPR)传感器芯片，该芯片由四个传感阵列组成，能够同时测量样品和控制传感器表面的结合事件。与基于ELISA的技术相比，SPR在无标记检测、实时测量和更高的灵敏度方面具有几个优势。用金传感器芯片通过EDC/NHS偶联的方法固定化α-酪蛋白多克隆抗体。首先对该分析和传感器的性能进行了优化，并在纯缓冲条件下进行了表征，作为直接结合分析，检测下限为58ngmL(-1)。采用夹心法和纳米颗粒扩增法可进一步提高检测灵敏度。然而，在这个阶段，这不是必需的，因为达到的检测限值超过了α-S1-酪蛋白的过敏原检测水平2微克毫升(-1)。该传感器对α-酪蛋白作为目标分析物具有良好的选择性，并对CIP最终漂洗样品进行了充分的回收。该传感器将是监测清洁程序后过敏原水平的有用工具，提供更多数据，可能更好地为食品制造商做出的更广泛的食物过敏原风险管理决策(S)提供信息。特别是，这种传感器可能有助于验证或优化给定食品制造过程的清洁实践。(C)2016爱思唯尔B.V.保留所有权利。
A simple, sensitive and label-free optical sensor method was developed for allergens analysis using a casein as the biomarker for cow's milk detection, to be used directly in final rinse samples of cleaning in place systems (CIP) of food manufacturers. A Surface Plasmon Resonance (SPR) sensor chip consisting of four sensing arrays enabling the measurement of samples and control binding events simultaneously on the sensor surface was employed in this work. SPR offers several advantages in terms of label free detection, real time measurements and superior sensitivity when compared to ELISA based techniques. The gold sensor chip was used to immobilise a-casein-polyclonal antibody using EDC/NHS coupling procedure. The performance of the assay and the sensor was first optimised and characterised in pure buffer conditions giving a detection limit of 58 ng mL(-1) as a direct binding assay. The assay sensitivity can be further improved by using sandwich assay format and amplified with nanoparticles. However, at this stage this is not required as the detection limit achieved exceeded the required allergens detection levels of 2 mu g mL(-1) for alpha-S1-casein. The sensor demonstrated good selectivity towards the a-casein as the target analyte and adequate recoveries from CIP final rinse wash samples. The sensor would be useful tool for monitoring allergen levels after cleaning procedures, providing additional data that may better inform upon wider food allergen risk management decision(s) that are made by food manufacturer. In particular, this sensor could potentially help validate or optimise cleaning practices for a given food manufacturing process. (C) 2016 Elsevier B.V. All rights reserved.