Antifungal activity of constituents from the heartwood of Gmelina arborea:: Part 1.: Sensitive antifungal assay against Basidiomycetes
Antifungal activity of constituents from the heartwood of Gmelina arborea:: Part 1.: Sensitive antifungal assay against Basidiomycetes
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DOI：
10.1515/hf.2004.028
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发表时间：
2004-01-01
期刊：
影响因子：
2.4
通讯作者：
Nishida, A
中科院分区：
文献类型：
--
作者：
Kawamura, F;Ohara, S;Nishida, A
研究了来自马来西亚 Gmelina arborea 心材的成分对 Trametes versicolor 和 Fomitopsis palustris 的抗真菌活性。开发了一种针对担子菌抗真菌活性的灵敏生物测定系统，该系统使用分散有均质菌丝的培养基。心材中的乙酸乙酯可溶物对两种真菌均表现出最高的活性，尽管对沼泽镰孢菌的活性相当弱。通过乙酸乙酯可溶物的自传确定了对花斑癣菌具有抗真菌活性的斑点，并分离并鉴定了五种成分：(+)-7'-O-乙基木酚、(+)泡桐素、(+)gmelinol、(+)表叶素和(-)-β-谷甾醇。四种木酚素显示出抗真菌活性，而β-谷甾醇则没有。从抗真菌活性的比较可以得出结论，胡椒基核有助于木脂素的活性。在分离出的四种木脂素中，gmelinol 似乎是一种重要的抗真菌成分，因为它在 G. arborea 的心材中含量丰富。此外，还确认了这5种化合物共存所产生的协同作用。
Antifungal activity of constituents from the heartwood of the Malaysian Gmelina arborea against Trametes versicolor and Fomitopsis palustris was investigated. A sensitive bioassay system for antifungal activity against basidiomycetes was developed which uses a medium in which homogenized hyphae were dispersed. Ethyl acetate solubles from the heartwood showed the highest activity against both fungi, although the activity against F. palustris was quite weak. Spots exhibiting antifungal activity against T. versicolor were specified by autobiography of ethyl acetatesolubles, and five constituents were isolated and identified as (+)-7'-O-ethyl arboreol, (+)paulownin, (+)gmelinol, (+)epieudesmin and (-)- beta-sitosterol. The four lignans showed antifungal activity, whereas beta-sitosterol did not. From the comparison of antifungal activity, it was concluded that the piperonyl nucleus contributed to the activity of lignans. Of the four lignans isolated, gmelinol appeared to be an important antifungal constituent, since it was rich in the heartwood of G. arborea. Furthermore, the synergism by coexistence of these five compounds was confirmed.