Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment
Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment
复制标题
DOI：
10.1038/ncomms3494
复制
发表时间：
2013-10-01
影响因子：
16.6
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
--
作者：
Ichihashi, Norikazu;Usui, Kimihito;Yomo, Tetsuya
进化的能力是区分生物和非生物化合物的一个关键特征。完全由非生命分子构建可进化的细胞样系统一直是一个重大挑战。在这里，我们构建了一个可进化的人工细胞模型，从生化分子的组装。人工细胞模型包含人工基因组RNA，其通过其编码的RNA复制酶的翻译进行复制。我们使用该系统进行了长期（600代）复制实验，其中突变通过复制错误自发引入RNA，并且根据达尔文原则，高度可复制的突变体在群体中占主导地位。在进化过程中，基因组RNA逐渐加强其与翻译的复制酶的相互作用，从而获得与自私（寄生）RNA的竞争力。这项研究提供了第一个实验证据，证明复制系统可以通过达尔文进化在一个类似细胞的隔间中发展，即使在寄生复制因子的存在下。
The ability to evolve is a key characteristic that distinguishes living things from non-living chemical compounds. The construction of an evolvable cell-like system entirely from non-living molecules has been a major challenge. Here we construct an evolvable artificial cell model from an assembly of biochemical molecules. The artificial cell model contains artificial genomic RNA that replicates through the translation of its encoded RNA replicase. We perform a long-term (600-generation) replication experiment using this system, in which mutations are spontaneously introduced into the RNA by replication error, and highly replicable mutants dominate the population according to Darwinian principles. During evolution, the genomic RNA gradually reinforces its interaction with the translated replicase, thereby acquiring competitiveness against selfish (parasitic) RNAs. This study provides the first experimental evidence that replicating systems can be developed through Darwinian evolution in a cell-like compartment, even in the presence of parasitic replicators.