Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts
Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts
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DOI：
10.1016/j.febslet.2006.09.015
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发表时间：
2006-10-16
期刊：
影响因子：
3.5
通讯作者：
Jeong, Daewon
中科院分区：
文献类型：
--
作者：
Kim, Hyunsoo;Kim, Ick Young;Jeong, Daewon
为了证明细胞氧化还原状态在破骨细胞分化过程中减少，在破骨细胞骨吸收过程中进一步减少，我们分析了γ -谷氨酰基合成酶活性，谷胱甘肽合成速率限制酶，以及总谷胱甘肽和巯基。中度和重度氧化还原向更氧化的环境转移诱导破骨细胞生成的逐渐增加和减少。此外，严重的谷胱甘肽消耗会抑制骨吸收，而适度的谷胱甘肽补充会增强骨吸收。总之，我们的观察结果表明，氧化还原状态存在一个阈值，代表破骨细胞分化和功能的双相模式。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed gamma-glutamyleysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing bi-phasic patterns in osteoclast differentiation and function. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.