Age-related regional differences in cardiac nerve growth factor expression
Age-related regional differences in cardiac nerve growth factor expression
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心脏神经生长因子表达的年龄相关区域差异
DOI：
10.1007/s11357-011-9262-0
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
P. Schauerte
中科院分区：
文献类型：
--
作者：
E. Saygili;R. Kluttig;O. Rana;E. Saygılı;C. Gemein;M. Zink;G. Rackauskas;J. Weis;R. Schwinger;N. Marx;P. Schauerte
年龄已被确定为心血管疾病的独立危险因素。据报道，老年人的心脏自主神经系统发生了交感神经紧张增加的转变。神经生长因子（NGF）是增加心脏交感神经活动的主要神经营养因子。与年轻心肌细胞相比，老年心肌细胞中的 NGF 表达是否存在变化以及心脏中是否存在区域差异仍不清楚。因此，我们选择不同年龄的大鼠模型（3-4天 = 新生，6-8周 = 年轻，20-24个月 = 老年），并分离左心房和右心房（LA，RA）以及左心室和右心室（LV，RV）的心肌细胞，用于测定NGF mRNA和蛋白的表达水平。在新生、年轻和年老大鼠中，LA 和 RA 中的 NGF 含量显着低于 LV 和 RV。在年轻和年老大鼠中，我们发现 LA 中的 NGF 蛋白水平显着高于 RA。此外，在新生儿、年轻人和老年人年龄组之间，两个心房的 NGF 表达均有所增加。在两个心室中，我们观察到从新生大鼠到年轻大鼠的 NGF 表达显着下降，从年轻大鼠到老年大鼠的 NGF 表达显着增加。在新生大鼠中观察到左心室和右心室中 NGF 含量最高。关于酪氨酸激酶A受体（TrkA）的表达，NGF信号传导的主要受体，老年大鼠的两个心房都表现出最大的表达；而在LV和RV中，TrkA主要在幼龄大鼠中表达。这些结果表明神经生长因子对老年患者自主神经张力变化的贡献。
Age has been identified as an independent risk factor for cardiovascular diseases. A shift of the cardiac autonomic nervous system towards an increase in sympathetic tone has been reported in the elderly. Nerve growth factor (NGF) is the main neurotrophic factor that increases the sympathetic activity of the heart. If there is a shift of NGF expression in old compared to young cardiomyocytes and whether there are regional differences in the heart still remain unclear. Therefore, we chose a rat model of different-aged rats (3–4 days = neonatal, 6–8 weeks = young, 20–24 months = old), and isolated cardiomyocytes from the left and the right atrium (LA, RA), as well as from the left and the right ventricle (LV, RV), were used to determine NGF expression on mRNA and protein levels. In neonatal, young, and old rats, NGF amount in LA and RA was significantly lower as compared to LV and RV. In young and old rats, we found significant higher NGF protein levels in LA compared to RA. In addition, both atria showed an increase in NGF expression between age groups neonatal, young, and old. In both ventricles, we observed a significant decrease in NGF expression from neonatal to young rats and a significant increase from young to old rats. The highest NGF amount in LV and RV was observed in neonatal rats. Regarding tyrosine kinase A receptor (TrkA) expression, the main receptor for NGF signaling, both atria showed the largest expression in old rats; while in LV and RV, TrkA was expressed mainly in young rats. These results point to a contribution of nerve growth factors to the change of autonomic tone observed in elderly patients.
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影响因子：
2.7
作者：
HASSANKHANI, A;STEINHELPER, ME;FEDEROFF, HJ
通讯作者：
FEDEROFF, HJ
影响因子：
37.8
作者：
S. Franklin;William Gustin;N. Wong;M. Larson;M. Weber;W. Kannel;Daniel Levy
通讯作者：
S. Franklin;William Gustin;N. Wong;M. Larson;M. Weber;W. Kannel;Daniel Levy
影响因子：
10.8
作者：
Chen, PS;Chen, LS;Fishbein, MC
通讯作者：
Fishbein, MC
影响因子：
158.5
作者：
Olivetti, G;Abbi, R;Anversa, P
通讯作者：
Anversa, P