A novel human SCAN/(Cys)2(His)2 zinc-finger transcription factor ZNF323 in early human embryonic development
A novel human SCAN/(Cys)2(His)2 zinc-finger transcription factor ZNF323 in early human embryonic development
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DOI：
10.1016/s0006-291x(02)00772-6
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发表时间：
2002-08-09
影响因子：
3.1
通讯作者：
Liu, MY
中科院分区：
文献类型：
--
作者：
Pi, HL;Li, YQ;Liu, MY
在许多转录因子中发现的C2H2锌指基序被认为对核酸结合和/或二聚化很重要。在这里，我们利用来自早期人类胚胎心脏cDNA文库的简并引物，鉴定并表征了一个新的锌指基因ZNF323。预测的蛋白质含有六种不同的C2H2型锌指和一个扫描盒。ZNF323定位于染色体6p22.1-22.3。15 ~ 23周人胚胎不同发育阶段表达水平不同。Northern blot分析显示，一个3.2 kb的ZNF323特异性转录本在肺、肝和肾中高表达，而在肠、脑、肌肉、胆囊、心脏和胰腺中表达较弱。在成人组织中，ZNF323在肝脏和肾脏中高表达，在肺、胰腺、脑、胎盘、肌肉和心脏中表达较弱。综上所述，这些结果表明ZNF323是锌指转录因子家族的成员，可能参与了多个胚胎器官的发育。(C) 2002 Elsevier Science （USA）。版权所有。
The C2H2 zinc-finger motif found in many transcription factors is thought to be important for nucleic acid binding and/or dimerization. Here, we have identified and characterized a novel zinc-finger gene named ZNF323 using degenerate primers from an early human embryo heart cDNA library. The predicted protein contains six different C2H2, type zinc fingers and a SCAN box. ZNF323 maps to chromosome 6p22.1-22.3. The expression levels were different during different development stages of human embryo between 15 and 23 weeks. Northern blot analysis shows that a 3.2-kb transcript specific for ZNF323 was expressed at high levels in the lung, liver, and kidney, while weakly expressed in intestine, brain, muscle, cholecyst, heart, and pancreas. In adult tissues, ZNF323 is expressed at high levels in liver and kidney, weakly in lung, pancreas, brain, placenta, muscle, and heart. Taken together, these results indicate that ZNF323 is a member of the zinc-finger transcription factor family and may be involved in the development of multiple embryonic organs. (C) 2002 Elsevier Science (USA). All rights reserved.