A small heat shock protein 21 (sHSP21) mediates immune responses in Chinese oak silkworm Antheraea pernyi
A small heat shock protein 21 (sHSP21) mediates immune responses in Chinese oak silkworm Antheraea pernyi
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DOI：
10.1016/j.ijbiomac.2018.01.147
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发表时间：
2018-05-01
影响因子：
8.2
通讯作者：
Tang, Bo-Ping
中科院分区：
文献类型：
--
作者：
Liu, Qiu-Ning;Liu, Yu;Tang, Bo-Ping
小分子热休克蛋白(SHSPs)在昆虫中高度保守，在温度胁迫、非生物胁迫、免疫应答、变态和胚胎发育等多种生物学过程中发挥重要作用。柞蚕是一种经济价值很高的产丝蛾，也是含有优质蛋白质的昆虫食物来源。本研究的目的是定量表达ApsHSP21基因对病原体相关分子模式(PAMPs)和核型多角体病毒(NPV)攻击的响应。推导的ApsHSP21蛋白序列由186个残基组成，分子量为21.0 kDa，等电子点为6.63。该蛋白含有一个保守的α-晶体蛋白结构域(ACD)，包括两个酪蛋白激酶II磷酸化位点、一个蛋白激酶C磷酸化位点、两个酪氨酸激酶磷酸化位点和多种多肽结合位点。系统发育分析表明，ApsHSP21与其他昆虫的同源物关系密切。实时定量逆转录聚合酶链式反应(qRT-PCR)分析表明，在模拟病原菌经脂多糖(LPS)、肽聚糖(PGN)、葡聚糖和NPV攻击后的不同时间点，ApsHSP21的表达显著上调。这些结果表明，sHSP21参与了Pernyi的先天免疫反应。(C)2018爱思唯尔B.V.保留所有权利。
Small heat shock proteins (sHSPs) are conserved among insects and play an important role in the regulation of many biological processes, including temperature stress, abiotic stress, immune responses, metamorphosis, and embryo development. Antheraea pernyi is an economically valuable silk-producing moth and source of insect food containing high-quality protein. The aim of this study was to quantify expression of the ApsHSP21 gene in response to pathogen-associated molecular patterns (PAMPs) and nucleopolyhedrovirus (NPV) challenge. The deduced ApsHSP21 protein sequence consists of 186 residues with a calculated molecular mass of 21.0 kDa and an isoelectronic point (pI) of 6.63. The protein contains a conserved alpha-crystallin domain (ACD), and includes two casein kinase II phosphorylation sites, a protein kinase C phosphorylation site, two tyrosine kinase phosphorylation sites, and various polypeptide binding sites. Phylogenetic analysis revealed that ApsHSP21 is closely related to homologs from other insects. Real-time quantitative reverse transcription PCR (qRT-PCR) analysis revealed that expression of ApsHSP21 was significantly up-regulated at different timepoints following simulated pathogen challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), glucan, and NPV. The results suggest sHSP21 is involved in innate immune responses in A. pernyi. (C) 2018 Elsevier B.V. All rights reserved.