pH-Driven Actuation of DNA Origami via Parallel I-Motif Sequences in Solution and on Surfaces
pH-Driven Actuation of DNA Origami via Parallel I-Motif Sequences in Solution and on Surfaces
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DOI：
10.1021/acs.bioconjchem.7b00288
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发表时间：
2017-07-01
影响因子：
4.7
通讯作者：
LaBean, Thomas H.
中科院分区：
文献类型：
--
作者：
Majikes, Jacob M.;Ferraz, Lucas C. C.;LaBean, Thomas H.
由于自底向上的DNA纳米制造创造了越来越复杂和动态的机制，在DNA纳米技术工具包中执行器的实现变得越来越重要。其中一个驱动器，I-motif，相当简单，因为它仅由标准DNA序列组成，不需要任何修饰化学或特殊纯化，而不是典型的DNA低聚物合成。本研究提出了一种新的并行I-motif驱动器，强调了它们作为子结构之间复杂内部运动驱动器的未来潜力。在这里，我们通过AFM和图像分析表征DNA折纸子结构之间的内部运动。这种平行i基序设计和驱动的量化为更复杂和有效的分子机器提供了有用的一步。
As bottom up DNA nanofabrication creates increasingly complex and dynamic mechanisms, the implementation of actuators within the DNA nanotechnology toolkit has grown increasingly important. One such actuator, the I-motif, is fairly simple in that it consists solely of standard DNA sequences and does not require any modification chemistry or special purification beyond that typical for DNA oligomer synthesis. This study presents a new implementation of parallel I-motif actuators, emphasizing their future potential as drivers of complex internal motion between substructures. Here we characterize internal motion between DNA origami substructures via AFM and image analysis. Such parallel I-motif design and quantification of actuation provide a useful step toward more complex and effective molecular machines.