Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo
Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo
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DOI：
10.1016/j.ydbio.2008.01.038
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发表时间：
2008-04-15
影响因子：
2.7
通讯作者：
Affolter, Markus
中科院分区：
文献类型：
--
作者：
Blum, Yannick;Belting, Heinz-Georg;Affolter, Markus
斑马鱼胚胎段间血管（ISV）的形成是研究体内血管生成的一个范例。ISV的形成被认为是在离散的步骤中发生的。首先，背侧主动脉的内皮细胞迁移出来并沿背腹轴沿着。最背侧的细胞，也被称为尖端细胞，然后与它的前后邻居连接，从而建立一个简单的维管网络。然后通过形成空泡建立血管腔，空泡最终与相邻内皮细胞的空泡融合以产生具有细胞内腔的无缝管。为了详细研究细胞结构和ISV的发展，我们分析了内皮细胞连接的排列，并进行了单细胞活体成像。与以前的报道相反，我们发现内皮细胞不是以线性的头到尾的结构排列，而是广泛重叠并形成一个多细胞管，其中包含一个细胞外腔。我们的研究表明，一些细胞的行为，如细胞分裂，细胞重排和动态变化的细胞-细胞接触，必须考虑在研究的形态和分子过程中参与ISV和内皮管腔形成在体内。(C)2008年爱思唯尔公司All rights reserved.
The formation of intersegmental blood vessels (ISVs) in the zebrafish embryo serves as a paradigm to study angiogenesis in vivo. ISV formation is thought to occur in discrete steps. First, endothelial cells of the dorsal aorta migrate out and align along the dorsoventral axis. The dorsal-most cell, also called tip cell, then joins with its anterior and posterior neighbours, thus establishing a simple vascular network. The vascular lumen is then established via formation of vacuoles, which eventually fuse with those of adjacent endothelial cells to generate a seamless tube with an intracellular lumen. To investigate the cellular architecture and the development of ISVs in detail, we have analysed the arrangement of endothelial cell junctions and have performed single cell live imaging. In contrast to previous reports, we find that endothelial cells are not arranged in a linear head-to-tail configuration but overlap extensively and form a multicellular tube, which contains an extracellular lumen. Our studies demonstrate that a number of cellular behaviours, such as cell divisions, cell rearrangements and dynamic alterations in cell-cell contacts, have to be considered when studying the morphological and molecular processes involved in ISV and endothelial lumen formation in vivo. (C) 2008 Elsevier Inc. All rights reserved.