Detection of gastric cancer micrometastases in lymph nodes by amplification of keratin 19 mRNA with reverse transcriptase polymerase chain reaction
Detection of gastric cancer micrometastases in lymph nodes by amplification of keratin 19 mRNA with reverse transcriptase polymerase chain reaction
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DOI：
10.1111/j.1349-7006.1996.tb00272.x
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发表时间：
1996-06-01
期刊：
影响因子：
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通讯作者：
Iwanaga, T
中科院分区：
文献类型：
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作者：
Noguchi, S;Hiratsuka, M;Iwanaga, T
建立了一种灵敏的检测胃癌淋巴结微转移的方法。该方法基于通过逆转录酶-聚合酶链反应（RT-PCR）扩增角蛋白19 mRNA。角蛋白19 RT-PCR结果显示12例胃癌组织中均有角蛋白19 mRNA表达，而20例正常对照淋巴结中均无角蛋白19 mRNA表达，提示角蛋白19 mRNA是检测胃癌淋巴结微转移的良好靶点。从胃癌中提取的RNA与从对照淋巴结中提取的RNA的系列稀释研究表明，角蛋白19 RT-PCR方法的检测灵敏度为1个癌细胞/10（3）-10（5）个淋巴结细胞。采用12例胃癌患者的100个淋巴结，比较了角蛋白19 RT-PCR和常规组织学检查对淋巴结转移的检出率。角蛋白19 mRNA在所有7个组织学转移阳性的淋巴结中检测到。在93个组织学转移阴性的淋巴结中，发现79个不表达角蛋白19 mRNA，但发现14个表达角蛋白19 mRNA，表明这些淋巴结含有组织学检查无法检测到的微转移。这些结果表明，角蛋白19 RT-PCR是一种比组织学检查更敏感的检测胃淋巴结微转移的方法。
A sensitive method for the detection of gastric cancer micrometastases in lymph nodes was developed. The method was based on amplification of keratin 19 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Keratin 19 RT-PCR showed that keratin 19 mRNA was expressed in all 12 gastric cancers, but not in any of 20 normal control lymph nodes, indicating that keratin 19 mRNA is a good target of RT-PCR for the detection of gastric cancer micrometastases in lymph nodes. Serial dilution studies of RNA extracted from gastric cancers against RNA extracted from control lymph nodes demonstrated that the detection sensitivity of the keratin 19 RT-PCR method was one cancer cell in 10(3)-10(5) lymph node cells. Detectability of lymph node metastases was compared between keratin 19 RT-PCR and conventional histological examination, using 100 lymph nodes obtained from 12 gastric cancer patients. Keratin 19 mRNA was detected in all of the seven lymph nodes which were histologically metastasis-positive. Of the 93 lymph nodes which were histologically metastasis-negative, 79 were found not to express keratin 19 mRNA but 14 were found to express keratin 19 mRNA, indicating that these lymph nodes contained micrometastases which could not be detected by histological examination. These results demonstrate that keratin 19 RT-PCR is a more sensitive method than histological examination for the detection of gastric micrometastases in lymph nodes.