Identification of genes related to skin development in potato
Identification of genes related to skin development in potato
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DOI：
10.1007/s11103-017-0619-3
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发表时间：
2017-07-01
影响因子：
5.1
通讯作者：
Ginzberg, Idit
中科院分区：
文献类型：
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作者：
Vulavala, Vijaya K. R.;Fogelman, Edna;Ginzberg, Idit
微阵列被用来比较马铃薯的皮肤和块茎肉转录本，以确定影响皮肤作为保护组织的独特特征的基因。功能基因分析表明，参与细胞分裂、细胞分化、形态发生和次生细胞壁形成(木质化和木质化)等发育过程以及与胁迫相关的活动的基因在块茎皮中的表达高于块茎肉。选择了几个在皮肤中差异表达的基因(经qPCR验证)，并选择了几个以前在马铃薯中没有发现的基因进行进一步分析。这些基因包括StKCS20-like、StFAR3、StCYP86A22和StPOD72-like基因，它们的序列表明它们可能与已知的Suberin相关基因密切相关；指导分生组织特异表达的StHAP3转录因子；以及StCASP1B2和StCASP1-like基因，它们是一个蛋白质家族的两个同源基因，介导拟南芥根部内胚层Casparian条带的形成。对携带这些基因的GUS报告结构的转基因植物诱导的微块茎的检测表明，这些基因在皮肤中表达，在块茎肉中很少或根本没有表达。一些报告基因在茎的内层、根内胚层、维管形成层和表皮中优先表达。相应启动子序列中的顺式调控元件支持这种基因表达模式。
Microarrays were used to compare the skin and tuber-flesh transcriptomes of potato, to identify genes that contribute to the unique characteristics of the skin as a protective tissue. Functional gene analysis indicated that genes involved in developmental processes such as cell division, cell differentiation, morphogenesis and secondary cell wall formation (lignification and suberization), and stress-related activities, are more highly expressed in the skin than in the tuber flesh. Several genes that were differentially expressed in the skin (as verified by qPCR) and had not been previously identified in potato were selected for further analysis. These included the StKCS20-like, StFAR3, StCYP86A22 and StPOD72-like genes, whose sequences suggest that they may be closely related to known suberin-related genes; the StHAP3 transcription factor that directs meristem-specific expression; and the StCASP1B2-like and StCASP1-like genes, which are two orthologs of a protein family that mediates the formation of Casparian strips in the suberized endodermis of Arabidopsis roots. An examination of microtubers induced from transgenic plants carrying GUS reporter constructs of these genes indicated that these genes were expressed in the skin, with little to no expression in the tuber flesh. Some of the reporter constructs were preferentially expressed in the inner layers of the skin, the root endodermis, the vascular cambium and the epidermis of the stem. Cis-regulatory elements within the respective promoter sequences support this gene-expression pattern.