MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients
MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients
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DOI：
10.1034/j.1399-3003.2000.01511.x
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发表时间：
2000-06-01
影响因子：
24.3
通讯作者：
Luisetti, M
中科院分区：
文献类型：
--
作者：
Viglio, S;Iadarola, P;Luisetti, M
细胞外基质成分的降解是慢性破坏性肺疾病许多病理特征的核心。桥连素和异桥连素是一种弹性蛋白衍生的交联型氨基酸，其尿液水平被认为是弹性蛋白降解的代表。本研究的目的是应用一种新的方法，基于高效毛细管电泳法，对11例稳定期慢性阻塞性肺疾病(COPD)患者、10例COPD加重患者、9例α(1)-抗胰蛋白酶缺乏症患者、13例支气管扩张患者和11例成人囊性纤维化患者的尿桥桥素和异桥桥素进行定量检测，并与24例正常对照组进行比较。结果发现，COPD稳定期患者尿桥桥素水平高于正常对照组(P=0.03)，但低于COPD急性加重组(p<0.05)。α(1)-抗胰蛋白酶缺乏症、支气管扩张和囊性纤维化患者的桥粒蛋白水平最高(p
Degradation of extracellular matrix components is central to many pathological features of chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross-linked amino acids whose urine levels are considered representative of elastin breakdown.The aim of this study was to apply a novel methodology, based on high-performance capillary electrophoresis, to the quantification of desmosine and isodesmosine in 11 patients with stable chronic obstructive pulmonary disease (COPD), 10 with an exacerbation of COPD, nine with alpha(1)-antitrypsin deficiency, 13 with bronchiectasis, and 11 adults with cystic fibrosis, in comparison to 24 controls.It was found that, in patients with stable COPD, urinary desmosine levels were higher than in controls (p=0.03), but lower than in COPD subjects with an exacerbation (p less than or equal to 0.05). The highest desmosine levels were found in subjects with alpha(1)-antitrypsin deficiency, bronchiectasis and cystic fibrosis (p