Phylogeographic relationships in the polypore fungus Pycnoporus inferred from molecular data.
Phylogeographic relationships in the polypore fungus Pycnoporus inferred from molecular data.
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DOI：
10.1111/j.1574-6968.2011.02412.x
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发表时间：
2011-12
影响因子：
2.1
通讯作者：
L. Lesage-Meessen;M. Haon;E. Uzan;A. Levasseur;F. Piumi;D. Navarro;Sabine Taussac;A. Favel;
中科院分区：
文献类型：
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作者：
L. Lesage-Meessen;M. Haon;E. Uzan;A. Levasseur;F. Piumi;D. Navarro;Sabine Taussac;A. Favel;
密孔菌属（Pycnoporus）形成了一组四个物种，已知它们尤其能产生适用于白色生物技术的高氧化还原电位漆酶。对来自不同地理区域的36株密孔菌进行了研究，以寻求在实验室培养条件下对新菌株进行分型的信息分子标记，并分析这一世界性群体的地理关系。对ITS 1 -5. 8 S-ITS 2核糖体DNA及β-微管蛋白和漆酶lac 3 -1基因的部分区域进行了测序。从这些序列推断的系统发育树明确区分Pycnoporus cinnabarinus菌株组的Pycnoporus puniceus菌株到强烈支持的分支（100%的引导值）。基于lac 3-1序列的分子聚类使Pycnoporus sanguineus和Pycnoporus coccineus通过四个不同的，支持良好的分支和亚分支的分布。一个新热带亚分支，将来自法属圭亚那和委内瑞拉的P. sanguineus菌株分组，对应于严格意义上的P. sanguineus。一个古热带亚分支，聚集了来自马达加斯加，越南和新喀里多尼亚的菌株，被定义为Pycnoporus cf。多血的澳大利亚分支对应于严格意义上的P. coccineus。东亚地区的分支，聚集了来自中国和日本的菌株，形成了一个类似P. coccineus的群体。密孔菌属漆酶基因（lac 3-1）的分析可以突出与地理起源相关的酶功能多样性。
The genus Pycnoporus forms a group of four species known especially for producing high redox potential laccases suitable for white biotechnology. A sample of 36 Pycnoporus strains originating from different geographical areas was studied to seek informative molecular markers for the typing of new strains in laboratory culture conditions and to analyse the phylogeographic relationships in this cosmopolitan group. ITS1-5.8S-ITS2 ribosomal DNA and partial regions of β-tubulin and laccase lac3-1 gene were sequenced. Phylogenetic trees inferred from these sequences clearly differentiated the group of Pycnoporus cinnabarinus strains from the group of Pycnoporus puniceus strains into strongly supported clades (100% bootstrap value). Molecular clustering based on lac 3-1 sequences enabled the distribution of Pycnoporus sanguineus and Pycnoporus coccineus through four distinct, well supported clades and sub-clades. A neotropical sub-clade, grouping the P. sanguineus strains from French Guiana and Venezuela, corresponded to P. sanguineus sensu stricto. A paleotropical sub-clade, clustering the strains from Madagascar, Vietnam and New Caledonia, was defined as Pycnoporus cf. sanguineus. The Australian clade corresponded to P. coccineus sensu stricto. The Eastern Asian region clade, clustering the strains from China and Japan, formed a P. coccineus-like group. Laccase gene (lac 3-1) analysis within the Pycnoporus species can highlight enzyme functional diversity associated with biogeographical origin.