Differential production of inflammatory chemokines by murine dendritic cell subsets
Differential production of inflammatory chemokines by murine dendritic cell subsets
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DOI：
10.1016/j.imbio.2004.03.002
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发表时间：
2004-01-01
期刊：
影响因子：
2.8
通讯作者：
Lahoud, MH
中科院分区：
文献类型：
--
作者：
Proietto, AI;O'Keeffe, M;Lahoud, MH
树突状细胞（DC）是具有激活初始T细胞能力的有效抗原呈递细胞。鼠DC代表可细分为不同亚群的异质性群体，包括常规DC（cDC）（其为CD 4（-）CD 8（-）（DN）、CD 4（+）CD 8（-）（CD 4（+））或CD 4（-）CD 8（+）（CD 8（+））亚群）和浆细胞样DC（pDC），其具有不同的免疫调节功能。在这项研究中，我们研究了编码炎性趋化因子Mip-1 α，Mip-1 β和Rantes的基因的差异表达，以及这些趋化因子的分泌，在脾脏DC亚群中。与CD 8（+）cDC相比，在体内静息和活化状态下，脾CD 4（+）和DN cDC亚群以更高水平表达这些趋化因子基因。pDC和cDC亚群均显示出响应于体外一系列toll样受体（TLR）刺激的趋化因子分泌增加。虽然pDC是响应于一些TLR刺激的Mip-1 α和Mip-1 β的最高生产者，但DN和CD 4（+）cDC亚群是Rantes的上级生产者。总体而言，在cDC中，CD 4（+）cDC最有效地产生所有趋化因子，无论是在基础水平，还是对大多数TLR刺激的反应。因此，我们报道了小鼠脾脏cDC亚群之间的新功能差异，其中CD 4（+）cDC证明了炎性趋化因子Mip-1 α、Mip-1 β和Rantes的最有效产生。(C)2004年Elsevier GmbH。All rights reserved.
Dendritic cells (DC) are efficient antigen presenting cells with the ability to activate naive T cells. Murine DC represent a heterogeneous population that can be subdivided into distinct subsets, including the conventional DC (cDC) which are either CD4(-)CD8(-) (DN), CD4(+)CD8(-) (CD4(+)) or CD4(-)CD8(+) (CD8(+)) subsets and the plasmacytoid DC (pDC), which have different immune regulatory functions. In this study, we investigated the differential expression of genes encoding the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes, and the secretion of these chemokines, among splenic DC subsets. These chemokine genes were expressed at higher levels by the splenic CD4(+) and DN cDC subsets compared with the CD8(+) cDC, in both the resting and activated states in vivo. Both the pDC and cDC subsets displayed increases in chemokine secretion in response to a range of toll-like receptor (TLR) stimuli in vitro. Whilst the pDC were the highest producers of Mip-1alpha and Mip-1beta in response to some TLR stimuli, the DN and CD4(+) cDC subsets were the superior producers of Rantes. Overall, of the cDC, the CD4(+) cDC produced all chemokines most efficiently, both at a basal level, and in response to most TLR stimuli. Thus, we report a new functional difference between the murine splenic cDC subsets, with the CD4(+) cDC demonstrating the most efficient production of the inflammatory chemokines Mip-1alpha, Mip-1beta and Rantes. (C) 2004 Elsevier GmbH. All rights reserved.