Production of cell‐enclosing hollow‐core agarose microcapsules via jetting in water‐immiscible liquid paraffin and formation of embryoid body‐like spherical tissues from mouse ES cells enclosed within these microcapsules
Production of cell‐enclosing hollow‐core agarose microcapsules via jetting in water‐immiscible liquid paraffin and formation of embryoid body‐like spherical tissues from mouse ES cells enclosed within these microcapsules
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DOI：
10.1002/bit.21624
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发表时间：
2008
影响因子：
3.8
通讯作者：
S. Sakai;I. Hashimoto;K. Kawakami
中科院分区：
文献类型：
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作者：
S. Sakai;I. Hashimoto;K. Kawakami
我们使用喷射技术开发了具有由藻酸盐微粒模板化的单个空心核心的琼脂糖微胶囊。我们将含有悬浮的藻酸盐微粒的琼脂糖水溶液挤出到液体石蜡的同向流中，并通过改变液体石蜡的流速来控制琼脂糖微粒的直径。随后使用藻酸盐裂解酶降解内部藻酸盐微粒导致空心结构。我们成功地获得了琼脂糖微胶囊，琼脂糖凝胶层厚度为20 - 50 μ m，空心直径为约100 μ m。50至450 µ m。使用藻酸盐微粒的ca。150 μ m直径和封闭猫肾细胞，我们能够创建细胞封闭琼脂糖微胶囊与中空核心约。直径150 µ m。这些微胶囊中的细胞比藻酸盐微粒中的细胞生长得快得多。此外，我们将小鼠胚胎干细胞封闭在琼脂糖微胶囊中。胚胎干细胞在包封后立即开始在核心中自聚集，随后在微囊的中空核心中生长并形成胚状体样球形组织。这些结果表明，我们的新的微胶囊生产技术和所得的微胶囊具有潜在的组织工程，细胞治疗和生物制药应用。Biotechnol. Bioeng. 2008; 99：235 - 243.© 2007 Wiley Periodicals，Inc.
We developed agarose microcapsules with a single hollow core templated by alginate microparticles using a jet‐technique. We extruded an agarose aqueous solution containing suspended alginate microparticles into a coflowing stream of liquid paraffin and controlled the diameter of the agarose microparticles by changing the flow rate of the liquid paraffin. Subsequent degradation of the inner alginate microparticles using alginate lyase resulted in the hollow‐core structure. We successfully obtained agarose microcapsules with 20–50 µm of agarose gel layer thickness and hollow cores ranging in diameter from ca. 50 to 450 µm. Using alginate microparticles of ca. 150 µm in diameter and enclosing feline kidney cells, we were able to create cell‐enclosing agarose microcapsules with a hollow core of ca. 150 µm in diameter. The cells in these microcapsules grew much faster than those in alginate microparticles. In addition, we enclosed mouse embryonic stem cells in agarose microcapsules. The embryonic stem cells began to self‐aggregate in the core just after encapsulation, and subsequently grew and formed embryoid body‐like spherical tissues in the hollow core of the microcapsules. These results show that our novel microcapsule production technique and the resultant microcapsules have potential for tissue engineering, cell therapy and biopharmaceutical applications. Biotechnol. Bioeng. 2008;99: 235–243. © 2007 Wiley Periodicals, Inc.